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EMILIN1 antibody

Principal name

EMILIN1 antibody

Alternative names for EMILIN1 antibody

EMILIN-1, EMI, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1

SwissProt ID

Q99K41 (Mouse), Q9Y6C2 (Human)

Gene ID

11117 (EMILIN1), 100952 (Emilin1)

Ncbi ID

9606, NP_008977, NP_598679, NP_008977.1, NP_598679.1, NM_133918, NM_007046

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of EMILIN1 antibody

Kit Component:: - KN305197G1, Emilin1 gRNA vector 1 in pCas-Guide vector
- KN305197G2, Emilin1 gRNA vector 2 in pCas-Guide vector
- KN305197D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

Western Blot analysis of EMILIN1 expression in transfected 293T cell line by EMILIN1 monoclonal antibody (M01), clone 4A3.Lane 1: EMILIN1 transfected lysate(35.9 KDa).Lane 2: Non-transfected lysate.

H00011117-M01

Detection limit for recombinant GST tagged EMILIN1 is approximately 0.3ng/ml as a capture antibody.

H00011117-M01

Western Blot analysis of EMILIN1 expression in transfected 293T cell line (H00011117-T01) by EMILIN1 MaxPab polyclonal antibody.Lane 1: EMILIN1 transfected lysate(38.17 KDa).Lane 2: Non-transfected lysate.

H00011117-B01P

Flow Cytometric analysis of 293 cells using EMILIN1 Antibody (N-term) (Cat.-No AP17327PU-N) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP17327PU-N

Formalin-fixed and paraffin-embedded human skeletal muscle with EMILIN1 Antibody (N-term) (Cat.-No AP17327PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

AP17327PU-N

Western blot analysis of EMILIN1 antibody (N-term) (Cat.-No AP17327PU-N) in in K562 and 293 cell line lysates (35 µg/lane). EMILIN1 (arrow) was detected using the purified Pab.

AP17327PU-N

Immunoprecipitation of EMILIN1 transfected lysate using anti-EMILIN1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EMILIN1 MaxPab mouse polyclonal antibody (B01) (H00011117-B01).

H00011117-D01

Cryostat sections of normal Mouse tissues stained with anti-Emilin-1 antibodies. In all Mouse tissues and organs examined, Emilin-1 was uniformly distributed in the stroma. In the skin, Emilin-1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, it colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that Emilin-1 is distributed at the abluminal surfaces of LECs . In the lymph node, Emilin-1-positive fibers connecting LECs to the surrounding ECM are evident.