EHD2 antibody
Principal name
EHD2 antibody
Alternative names for EHD2 antibody
PAST2, EH domain-containing protein 2, PAST homolog 2
Ncbi ID
9606, NP_055416.2, NP_055416, NP_694708.2, BC027084, NM_153068, NM_001024897, NM_014601
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of EHD2 antibody
- Kit Component:
- - KN305073G1, Ehd2 gRNA vector 1 in pCas-Guide vector
- KN305073G2, Ehd2 gRNA vector 2 in pCas-Guide vector
- KN305073D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8.Lane 1: EHD2 transfected lysate(61.2 KDa).Lane 2: Non-transfected lysate.
EHD2 monoclonal antibody (M01), clone 2D8 Western Blot analysis of EHD2 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged EHD2 is approximately 0.03ng/ml as a capture antibody.
EHD2 polyclonal antibody ( Cat # PAB6684 ) staining ( 0.003 µg/ml ) of human heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing EHD2 and probed with EHD2 polyclonal antibody ( Cat # PAB6684 ) ( mock transfection in first lane ), tested by Origene.
EHD2 monoclonal antibody (M02), clone 1F1. Western Blot analysis of EHD2 expression in HeLa(Cat # L013V1 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EHD2 (RC204848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EHD2.
ELISA: EHD2 Antibody
TA302472 staining (0.003µg/ml) of human heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302472 | EHD2 (C-term) antibody |
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| Goat Polyclonal Antibody against EHD2 | |||||||
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Goat | Aff - Purified | Hu, Ms, Rt, Ze | WB |
0.1 mg /
$360.00
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| OriGene Technologies, Inc. | |||||||