EGR1 antibody
Principal name
EGR1 antibody
Alternative names for EGR1 antibody
Early growth response protein 1, EGR-1, Zif268, Zif-268, NGFI-A, AT225, ETR103, KROX-24, Protein Krox-24, Transcription factor Zif268, Nerve growth factor-induced protein A, Transcription factor ETR103
Ncbi ID
9606, NP_031939, NP_001955.1, NP_001955, NM_007913, ACE87567.1, NP_031939.1, NM_001964
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of EGR1 antibody
Egr proteins function in transcription regulatory activities surrounding cellular growth, differentiation and function. The deduced amino acid sequences of human Egr-2 and mouse Egr-1 are 92% identical in the zinc finger region but show no homology elsewhere. Egr-2 is a sequence-specific DNA-binding transcription factor that binds two specific DNA sites located in the promoter region of HoxA4 and localizes to the nucleus. Defects in the Egr-2 protein are a cause of congenital hypomyelination neuropathy (CHN). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness and very slow nerve conduction velocities. Mutations in the gene that encodes Egr-2 (EGR2) also cause of Dejerine-Sottas syndrome (DSS), which is also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS patients exhibit severe early onset motor and sensory neuropathy with very slow nerve conduction velocities and elevated cerebrospinal fluid protein concentrations.
General readings
Schnell,F.J., et al., (2006) J. Immunol. 176 (8), 4740-4747
Detection limit for recombinant GST tagged EGR1 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to EGR1 on HeLa cell. [antibody concentration 10 ug/ml]
EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in Raw 264.7 ( Cat # L024V1 ). (82kD, J.L.R, http://www.jlr.org/cgi/content/full/43/9/1477)
Detection limit for recombinant GST tagged EGR1 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EGR1 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EGR1 is approximately 1ng/ml as a capture antibody.
EGR1 monoclonal antibody (M07), clone 3G3 Western Blot analysis of EGR1 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged EGR1 is approximately 0.3ng/ml as a capture antibody.
EGR1 polyclonal antibody (A01), Lot # TTB0060123QCS1 Western Blot analysis of EGR1 expression in NIH/3T3 ( Cat # L018V1 ).
EGR1 polyclonal antibody (A01), Lot # . Western Blot analysis of EGR1 expression in Raw 264.7.
EGR1 polyclonal antibody (A01), Lot # . Western Blot analysis of EGR1 expression in PC-12.
Xenopus Laevis; . Sample Type :. Frog brain. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Biotinylated goat anti-rabbit. Secondary Antibody Dilution :. 1:200. Color/Signal Descriptions :. Black: EGF1. Gene Name :. EGR1 a. Submitted by :. Eva Fischer, Colorado State University; EGR1 antibody - N-terminal region (ARP36841_T100) in Xenopus Laevis cells using Immunohistochemistry
Xenopus Laevis; . Sample Type :. Frog brain. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Biotinylated goat anti-rabbit. Secondary Antibody Dilution :. 1:200. Color/Signal Descriptions :. Black: EGF1. Gene Name :. EGR1 a. Submitted by :. Eva Fischer, Colorado State University; EGR1 antibody - N-terminal region (ARP36841_T100) in Xenopus Laevis cells using Immunohistochemistry
Mouse NIH-3T3; WB Suggested Anti-EGR1 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: NIH/3T3 cell lysate; EGR1 antibody - N-terminal region (ARP36841_T100) in Mouse NIH-3T3 cells using Western Blot
EGR1 monoclonal antibody (M06), clone 3C7 Western Blot analysis of EGR1 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged EGR1 is approximately 0.3ng/ml as a capture antibody.
Western blot analysis of Egr-1/2 Antibody Cat.-No AP06678PU-N at 1/500 dilution. Lane 1: HEK293T whole cell lysate (40 µg) Lane 2: PC12 whole cell lysate (40 µg) Lane 3: NIH-3T3 whole cell lysate (40 µg) Lane 3: Jurkat whole cell lysate(40 µg).
Lung small cell carcinoma: Formalin-Fixed Paraffin-Embedded (FFPE)
EGR1 monoclonal antibody (M08), clone 1F5 Western Blot analysis of EGR1 expression in 293 ( Cat # L026V1 ).
Human colon; Immunohistochemistry with Colon, myenteric plexus tissue at an antibody concentration of 5.0ug/ml using anti-EGR1 antibody (ARP32241_P050); EGR1 antibody - N-terminal region (ARP32241_P050) in Human colon cells using Immunohistochemistry
Human Intestine; Rabbit Anti-EGR1 Antibody. Catalog Number: ARP32241. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; EGR1 antibody - N-terminal region (ARP32241_P050) in Human Intestine cells using Immunohistochemistry
Human Spleen; Rabbit Anti-EGR1 Antibody. Catalog Number: ARP32241. Paraffin Embedded Tissue: Human Spleen. Cellular Data: Spleen cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; EGR1 antibody - N-terminal region (ARP32241_P050) in Human Spleen cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-EGR1 Antibody Titration: 1 ug/ml. Positive Control: Jurkat cell lysate; EGR1 antibody - N-terminal region (ARP32241_P050) in Human Jurkat cells using Western Blot
Immunohistochemistry of EGR1 polyclonal antibody ( Cat # PAB10007 ) was used at a 10 µg/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides.Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining.This image shows EGR1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Western blot using EGR1 polyclonal antibody ( Cat # PAB10007 ) shows detectionof a predominant band at ~58 kDa correspond-ing to EGR1 present in mouse embryonic fibroblast whole cell lysate ( arrowhead ).Approximately 35 µg of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1,500.Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Western blot analysis of EGR1 Antibody (N-term) (AP17311PU-N) in mouse NIH-3T3 tissue lysates (35 µg/lane). EGR1 (arrow) was detected using the purified Pab.
Human OVCAR-3; WB Suggested Anti-EGR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate; EGR1 antibody - middle region (ARP58382_P050) in Human OVCAR-3 cells using Western Blot
Xenopus Laevis; . Sample Type :. Frog brain. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Biotinylated goat anti-rabbit. Secondary Antibody Dilution :. 1:200. Color/Signal Descriptions :. Black: EGR1. Gene Name :. Egr1 a. Submitted by :. Eva Fischer, Colorado State University; Egr1 antibody - C-terminal region (ARP36840_P050) in Xenopus Laevis cells using Immunohistochemistry
Xenopus Laevis; . Sample Type :. Frog brain. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Biotinylated goat anti-rabbit. Secondary Antibody Dilution :. 1:200. Color/Signal Descriptions :. Black: EGR1. Gene Name :. Egr1 a. Submitted by :. Eva Fischer, Colorado State University; Egr1 antibody - C-terminal region (ARP36840_P050) in Xenopus Laevis cells using Immunohistochemistry
Mouse Heart; WB Suggested Anti-Egr1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Mouse Heart; Egr1 antibody - C-terminal region (ARP36840_P050) in Mouse Heart cells using Western Blot
Western blot analysis using EGR1 mouse mAb against EGR1(AA: 282-433)-hIgGFc transfected HEK293 (1)cell lysate.
Immunohistochemical analysis of EGR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of EGR1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of EGR1 expression in MCF7 (A); SHSY5Y (B); PC12 (C) whole cell lysates.
Western blot analysis of mouse brain lysate (95ug cell lysate) with anti-EGR1 antibody diluated at 1:2000. (Predicted band size: 58kDa; observed band size: 82kDa)
Western blot analysis of extracts from HeLa/Jurkat cells, using EGR1/2 antibody.The lane on the right is treated with the synthesized peptide.
WB using Anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Primary antibody was used at a 1,500 dilution. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.
Predicted band size: 58 kDa . Positive control: RAW264.7 and NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: RAW264.7 cell lysate Lane2: NIH/3T3 cell lysate Lysates: 50 ug per lane Primary antibody: 1/400dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
WB Suggested Anti-EGR1 Antibody Titration: 1 ug/ml; Positive Control: Jurkat cell lysate
Rabbit Anti-EGR1 Antibody; Paraffin Embedded Tissue: Human Spleen; Cellular Data: Spleen cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Rabbit Anti-EGR1 Antibody; Paraffin Embedded Tissue: Human Intestine; Cellular Data: Epithelial cells of intestinal villas; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Immunohistochemistry with Colon, myenteric plexus tissue at an antibody concentration of 5.0 ug/ml using anti-EGR1 antibody
WB Suggested Anti-Egr1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Mouse Heart
Sample Type . Frog brainPrimary Antibody Dilution . 1:500Secondary Antibody . Biotinylated goat anti-rabbitSecondary Antibody Dilution . 1:200Color/Signal Descriptions . Black: EGR1Gene Name . Egr1 aSubmitted by . Eva Fischer, Colorado State University
Sample Type . Frog brainPrimary Antibody Dilution . 1:500Secondary Antibody . Biotinylated goat anti-rabbitSecondary Antibody Dilution . 1:200Color/Signal Descriptions . Black: EGR1Gene Name . Egr1 aSubmitted by . Eva Fischer, Colorado State University
WB Suggested Anti-EGR1 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:12500; Positive Control: NIH/3T3 cell lysate
Sample Type . Frog brainPrimary Antibody Dilution . 1:500Secondary Antibody . Biotinylated goat anti-rabbitSecondary Antibody Dilution . 1:200Color/Signal Descriptions . Black: EGF1Gene Name . EGR1 aSubmitted by . Eva Fischer, Colorado State University
Sample Type . Frog brainPrimary Antibody Dilution . 1:500Secondary Antibody . Biotinylated goat anti-rabbitSecondary Antibody Dilution . 1:200Color/Signal Descriptions . Black: EGF1Gene Name . EGR1 aSubmitted by . Eva Fischer, Colorado State University
WB Suggested Anti-EGR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: OVCAR-3 cell lysate
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209956LP | EGR1 - human gene knockout kit via CRISPR |
||
 |
Human | Luciferase-Puro |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||