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Dynactin subunit 1 antibody

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Principal name

Dynactin subunit 1 antibody

Alternative names for Dynactin subunit 1 antibody

DCTN1, DAP-150, DP-150, p150-glued, p135, 150 kDa dynein-associated polypeptide

SwissProt ID

O08788 (Mouse), P28023 (Rat), P35458 (Chick), Q14203 (Human)

Gene ID

1639 (DCTN1), 29167 (Dctn1), 13191

Ncbi ID

9606, NP_004073, NM_001135040, NP_004073.2, NP_001177766.1, NP_077044.1, NP_031861.2, NM_007835, NM_023019, NM_001135041, NM_001190836, NM_001190837

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Dynactin subunit 1 antibody

This gene encodes the largest subunit of dyctin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dyctin binds to both microtubules and cytoplasmic dynein. Dyctin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Altertive splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spil and bulbar muscular atrophy (dSBMA). [provided by RefSeq]

PAB6249 - Dynactin subunit 1
DCTN1 polyclonal antibody ( Cat # PAB6249 ) ( 2.5µg/ml ) staining staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
PAB6249 - Dynactin subunit 1
DCTN1 polyclonal antibody ( Cat # PAB6249 ) staining ( 1 µg/ml ) of human testis lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302830 - Dynactin subunit 1
TA302830 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302830 - Dynactin subunit 1
TA302830 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
TA307941 - Dynactin subunit 1
Sample (30 ug of whole cell lysate). A: JurKat. 5% SDS PAGE. TA307941 diluted at 1:3000.
TA307941 - Dynactin subunit 1
Immunohistochemical analysis of paraffin-embedded OVCA, using dynactin 1(TA307941) antibody at 1:100 dilution.
TA307941 - Dynactin subunit 1
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DCTN1(TA307941) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
TA332431 - Dynactin subunit 1
Western blot analysis of extracts of various cell lines, using DCTN1 antibody.
TA332431 - Dynactin subunit 1
Immunohistochemistry of paraffin-embedded human esophageal cancer using DCTN1 antibody at dilution of 1:100 (200x lens).
TA340044 - Dynactin subunit 1
WB Suggested Anti-Dctn1 Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Thymus
TA340044 - Dynactin subunit 1
WB Suggested Anti-Dctn1 Antibody; Positive Control: Lane 1: 80ug rat brain extract; Primary Antibody Dilution: 1: 500; Secondary Antibody: IRDye 800 CW goat anti-rabbit from Li-COR Bioscience; Secondry Antibody Dilution: 1: 20, 000; Submitted by: Dr. Yuzh

2 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Dynactin subunit 1 antibody
  Rabbit polyclonal antibody to dynactin 1 (dynactin 1 (p150, glued homolog, Drosophila))  
Sample (30 ug of whole cell lysate). A: JurKat. 5% SDS PAGE. TA307941 diluted at 1:3000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / $415.00
  OriGene Technologies, Inc.
+2 additional images

Dynactin subunit 1 antibody
  Rabbit anti-DCTN1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using DCTN1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / $335.00
  OriGene Technologies, Inc.
+1 additional image

2 Item(s)

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Product Type
  1. Primary Antibody (2)
Clonality
  1. Polyclonal (2)
Target
  1. Dynactin subunit 1 (2)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  2. Paraffin Sections (P) (1)
  3. Western blot / Immunoblot (WB) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Images
  1. 1 image (2)
  2. 3+ images (1)
Images: Applications
  1. IHC (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunofluorescence (IF) (1)
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  1. OriGene Technologies (2)
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