DAG kinase beta antibody
Principal name
DAG kinase beta antibody
Alternative names for DAG kinase beta antibody
Diacylglycerol kinase beta, Diglyceride kinase beta, DGKB, DAGK2, KIAA0718
Ncbi ID
9606, NP_004071.1, NM_004080, NP_004071, NP_848796.2, NM_178681, NM_019304, NM_145695
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of DAG kinase beta antibody
- Kit Component:
- - KN304528G1, Dgkb gRNA vector 1 in pCas-Guide vector
- KN304528G2, Dgkb gRNA vector 2 in pCas-Guide vector
- KN304528D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKB polyclonal antibody ( Cat # PAB3348 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of DGKB polyclonal antibody ( Cat # PAB3348 ) in mouse liver tissue lysate. DGKB ( arrow ) was detected using the purified polyclonal antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-hDGKB-T787 Pab in mouse liver tissue lysate. hDGKB-T787(arrow) was detected using the purified Pab.
Western blot analysis of DGK beta expression in HT29 (A); MCF7 (B); HeLa (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKB (RC211233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DGKB monoclonal antibody.
Western blot analysis of extracts from HuvEC cells, using DGKB antibody.The lane on the right is treated with the synthesized peptide.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317461 | DAG kinase beta (transcript variant 1) |
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| Recombinant protein of human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||