CYP17A1 antibody
Principal name
CYP17A1 antibody
Alternative names for CYP17A1 antibody
Cytochrome P450 17A1, CYPXVII, P450-C17, Steroid 17-alpha-monooxygenase, CYP17, S17AH, P450c17, Steroid 17-alpha-hydroxylase/17,20 lyase
SwissProt ID
P05093 (Human), P05185 (Bovin), P11715 (Rat), P12394 (Chick), P19100 (Pig), P27786 (Mouse), Q29497 (Sheep), Q64410 (Cavpo), Q95328 (Horse), Q9GMC8 (Felca)
Ncbi ID
NP_000093, 9606, NP_000093.1, AAH63388.1, NM_007809, NM_012753, NP_031835.3
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX)
Background of CYP17A1 antibody
The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A, CYP2A, CYP2C, CYP2D, CYP4A14, CYP7A, CYP7B, CYP8B, CYP11A, CYP17A1,CYPI9 and CYP27A, based on sequence similarities. CYP17A (17α-hydroxylase/17,20-lyase) is important for the conversion of pregnenolone and progesterone to dehydroepiandrosterone (DHEA) and androstenedione. In this process, it catalyzes both the 17α-hydroxylation and the 17,20-lyase reaction.CYP17A1 is crucial during sexual development, both during fetal development and during puberty, and is intracellularly regulated by cAMP levels. Defects in the CYP17A1 gene, which encodes for the protein, may cause adrenal hyperplasia type V (AH-V) which is characterized by hypokalemia and hypertension.Male patients affected by AH-V do not undergo normal sexual differentiation and develop female external genitalia and do not undergo pubertal develoment.
Immunohistochemistry (IHC) analyzes of CYP17A1 antibody (Cat.-No.: AP06771PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of CYP17A1 antibody (Cat.-No.: AP06771PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:H9C2 whole cell lysate
Lane1:Hela whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:H9C2 whole cell lysate
CYP17A1 polyclonal antibody ( Cat # PAB7014 ) ( 0.3 µg/ml ) staining of human ovary lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical analysis of Cytochrome P450 17A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytochrome P450 17A1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytochrome P450 17A1 expression in HeLa (A); NIH3T3 (B); H9C2 (C); SW13 (D); ES2 (E) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503442)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
Flow cytometric Analysis of Hela cells, using anti-CYP17A1 antibody(TA503442),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-CYP17A1 antibody(TA503442),(Red), compared to a nonspecific negative control antibody,(Blue).
CYP17A1 Luminex Elisa with 5G10 Capture (TA600326) and 3D9 Detection (TA700326) Antibodies. Substrate used: Recombinant Human CYP17A1 (TP309042)
CYP17A1 Luminex Elisa with 9H4 Capture (TA600327) and 3D9 Detection (TA700326) Antibodies. Substrate used: Recombinant Human CYP17A1 (TP309042)
CYP17A1 Luminex Elisa with 5G10 Capture (TA600326) and 3D9 Detection (TA700326) Antibodies. Substrate used: Recombinant Human CYP17A1 (TP309042)
CYP17A1 Luminex Elisa with 9H4 Capture (TA600327) and 3D9 Detection (TA700326) Antibodies. Substrate used: Recombinant Human CYP17A1 (TP309042)
TA302444 (0.3µg/ml) staining of human ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from A549 cells, Jurkat cells and HeLa cells, using Cytochrome P450 17A1 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of anti-CYP17A1 Antibody (Center)(Cat.#TA325002) in K562 cell line lysates (35ug/lane). CYP17A1(arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of CYP17A1 Antibody (Center) (Cat. #TA325002) with Hela cell followed by Alexa Fluoræ´?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302444 | CYP17A1 antibody |
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| Goat Polyclonal Antibody against CYP17A1 | |||||||
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Goat | Aff - Purified | Can, Fe, Hu | P, WB |
0.1 mg /
$360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA311723 | CYP17A1 antibody |
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| Rabbit polyclonal Cytochrome P450 17A1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325002 | CYP17A1 antibody |
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| Rabbit polyclonal CYP17A1 Antibody (Center) | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP06771PU-N | CYP17A1 antibody |
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 |
Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 mg /
$370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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