Cyclin A2 antibody
Principal name
Cyclin A2 antibody
Alternative names for Cyclin A2 antibody
Cyclin-A2, CCNA2, CCN1, CCNA, Cyclin A
Ncbi ID
NP_001228, NP_033958, 9606, NP_001228.1, 9913, NP_033958.2, NM_001237, NM_009828
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)
Background of Cyclin A2 antibody
- Kit Component:
- - KN302803G1, Ccna2 gRNA vector 1 in pCas-Guide vector
- KN302803G2, Ccna2 gRNA vector 2 in pCas-Guide vector
- KN302803D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Bartolo, D. L. D., et al., Blood 111, 4731-4740 (2008).
2. Ciarallo, S., et al., Mol. Cell. Biol. 22, 2993-3002 (2002).
3. Sandhu, C., et al., Oncogene 19, 5314-5323 (2000).
4. Sandhu, C., et al., Cancer Res. 60, 2616-2622 (2000).
5. Slingerland, J. M., et al., Mol. Cell. Biol. 14, 3683-3694 (1994).
6. Adamczewski, J. P., et al., J. Virol. 67, 6551-6557 (1993)
Clone E67.1 is used in these references.
Western blot analysis of Cyclin A expression in Jurkat cells (Lane 1), Raji cells (Lane 2), HeLa cells (Lane 3), WR19L cells (Lane 4) and PC12 cells (Lane 5) using AM20281AF-N Cyclin A antibody.
Western blot analysis of Cyclin A expression in Jurkat (Lane 1), Raji (Lane 2), HeLa (Lane 3), WR19L (Lane 4) and PC12 (Lane 5) using AM20282AF-N Cyclin A antibody.
Immunoprecipitation of Cyclin A from Jurkat cells with AM20282AF-N (Lane 1) or Mouse IgG (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with biotinylated anti-Cyclin A monoclonal antibody (AM20282BT-N). Jurkat crude lysate was resolved in Lane 3.
Immunoprecipitation of Cyclin A from Jurkat cells with AM20282AF-N (Lane 1) or Mouse IgG2a Isotype Control (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with biotinylated anti-Cyclin A monoclonal antibody (AM20282BT-N).
Human Testis; Ccna2 antibody - C-terminal region (ARP30159_P050) validated by WB using testis; Ccna2 antibody - C-terminal region (ARP30159_P050) in Human Testis cells using Western Blot
Rat Muscle; WB Suggested Anti-Ccna2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Rat Muscle; Ccna2 antibody - C-terminal region (ARP30159_P050) in Rat Muscle cells using Western Blot
Immunofluorescence of purified MaxPab antibody to CCNA2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CCNA2 expression in transfected 293T cell line (H00000890-T01) by CCNA2 MaxPab polyclonal antibody.Lane 1: CCNA2 transfected lysate(47.52 KDa).Lane 2: Non-transfected lysate.
Western blot expression of Ccna2 polyclonal antibody ( Cat # PAB0817 ) in Hela whole cell lysate.
Western blot analysis of CCNA2 polyclonal antibody ( Cat # PAB2867 ) in HL-60 cell line lysates ( 35µg/lane ) . CCNA2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Immunohistochemical staining with CCNA2 polyclonal antibody ( Cat # PAB9996 ) was diluted 1 : 500 to detect CCNA2 in human testis tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using Cyclin A2 antibody (Cat.-No. AP06079PU-N).
Western blot (WB) analysis of Cyclin A antibody (Cat.-No.: AP06079PU-N) at 1/500 dilution
Lane1:HEK293T cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:Rat brain tissue lysate
Lane1:HEK293T cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:Rat brain tissue lysate
Western blot analysis of anti-CCNA2(N-term) Pab in HL60 cell line lysates (35ug/lane). CCNA2(arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CCNA2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of CCNA2 (arrow) using rabbit polyclonal CCNA2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCNA2 gene (Lane 2)
Human HepG2; WB Suggested Anti-CCNA2 Antibody Titration: 1ug/ml. Positive Control: HepG2 cell lysate. CCNA2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; CCNA2 antibody - C-terminal region (AVARP03045_T100) in Human HepG2 cells using Western Blot
Immunoprecipitation of CCNA2 transfected lysate using anti-CCNA2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CCNA2 MaxPab mouse polyclonal antibody (B01) (H00000890-B01).
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-cyclin A antibody (clone E23.1). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and chemiluminescence detection.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNA2 (RC211148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CCNA2 Polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: Cyclin A2 Antibody
Western Blot of Rabbit anti- Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 µg per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.
Immunohistochemistry of Rabbit Anti-Cyclin A antibody. Tissue: human testes tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Cyclin A antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Cyclin A is nuclear and cytoplasmic. Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.
WB Suggested Anti-CCNA2 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysateCCNA2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319146 | Cyclin A2 antibody |
||||||
| Rabbit polyclonal anti-Cyclin A antibody | |||||||
 |
Rabbit | Serum | Hu, Ms, Rt | E, P, WB |
0.1 ml /
$515.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||