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CROT antibody

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Principal name

CROT antibody

Alternative names for CROT antibody

COT, Peroxisomal carnitine O-octanoyltransferase

SwissProt ID

O19094 (Bovin), P11466 (Rat), Q9DC50 (Mouse), Q9UKG9 (Human)

Gene ID

54677 (CROT), 83842 (Crot), 74114

Ncbi ID

9606, NP_066974, NP_114193.1, NM_001143935, NP_066974.2, NP_076222.1, NM_023733, NM_031987, NM_021151

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Eq (Equine), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F)

Background of CROT antibody

Kit Component:
- KN303846G1, Crot gRNA vector 1 in pCas-Guide vector
- KN303846G2, Crot gRNA vector 2 in pCas-Guide vector
- KN303846D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Morillas,M., (2002) J. Biol. Chem. 277 (13), 11473-11480

H00054677-M01 - CROT
CROT monoclonal antibody (M01), clone 1A6. Western Blot analysis of CROT expression in NIH/3T3 ( Cat # L018V1 ).
H00054677-M01 - CROT
CROT monoclonal antibody (M01), clone 1A6. Western Blot analysis of CROT expression in Raw 264.7 ( Cat # L024V1 ).
H00054677-M01 - CROT
CROT monoclonal antibody (M01), clone 1A6. Western Blot analysis of CROT expression in PC-12 ( Cat # L012V1 ).
H00054677-M01 - CROT
CROT monoclonal antibody (M01), clone 1A6 Western Blot analysis of CROT expression in HeLa ( Cat # L013V1 ).
H00054677-A01 - CROT
CROT polyclonal antibody (A01), Lot # 051205JC01 Western Blot analysis of CROT expression in MES-SA/Dx5 ( Cat # L021V1 ).
H00054677-B01 - CROT
Western Blot analysis of CROT expression in transfected 293T cell line (H00054677-T01) by CROT MaxPab polyclonal antibody.Lane 1: CROT transfected lysate(67.32 KDa).Lane 2: Non-transfected lysate.
H00054677-D01P - CROT
Western Blot analysis of CROT expression in transfected 293T cell line (H00054677-T02) by CROT MaxPab polyclonal antibody.Lane 1: CROT transfected lysate(70.20 KDa).Lane 2: Non-transfected lysate.
ARP48873_T100 - CROT
Human HepG2; WB Suggested Anti-CROT Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; CROT antibody - N-terminal region (ARP48873_T100) in Human HepG2 cells using Western Blot
AP51088PU-N - CROT
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP51088PU-N - CROT
Western blot analysis of CROT Antibody (N-term) (Cat. #AP51088PU-N) in K562 cell line and mouse liver tissue lysates (35µg/lane). CROT (arrow) was detected using the purified Pab.
AP51088PU-N - CROT
CROT Antibody (N-term)(Cat. #AP51088PU-N) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP32804PU-N - CROT
Staining of Mouse Liver lysate using CROT Antibpdy Cat.-No AP32804PU-N  at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TP307888 - CROT
TA307959 - CROT
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA307959 diluted at 1:1000.
TA307959 - CROT
Sample (30 ug of whole cell lysate). A: 293T. B: A431 . 7.5% SDS PAGE. COT antibody. TA307959 diluted at 1:1000.
TA307959 - CROT
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CROT(TA307959) antibody at 1:100 dilution.
TA311543 - CROT
(0.3ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA349832 - CROT
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, mouse kidney tissue, Primary antibody: (CROT Antibody) at dilution 1/270, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
TA349832 - CROT
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (CROT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA349832 - CROT
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (CROT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA346810 - CROT
WB Suggested Anti-CROT Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CROT (178-199) antibody
Staining of Mouse Liver lysate using CROT Antibpdy Cat.-No AP32804PU-N  at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Ms, Rt E, WB
0.1 mg / $380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

CROT antibody
  Rabbit Polyclonal antibody to CROT (carnitine O-octanoyltransferase)  
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA307959 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / $415.00
  OriGene Technologies, Inc.
+2 additional images

CROT (C-term) antibody
  Goat Anti-Crot (rat) Antibody  
(0.3ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Rt E, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

CROT antibody
  Rabbit Polyclonal Anti-CROT Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, mouse kidney tissue, Primary antibody: (CROT Antibody) at dilution 1/270, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / $335.00
  OriGene Technologies, Inc.
+2 additional images

CROT (N-term) antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms F, P, WB
0.4 ml / $415.00
  OriGene Technologies GmbH
+2 additional images

5 Item(s)

per page

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Clonality
  1. Polyclonal (5)
Target
  1. CROT (5)
Property
  1. 178-199 (1)
  2. C-term (1)
  3. N-term (1)
Reactivity
  1. Mouse (Ms) (4)
  2. Human (Hu) (3)
  3. Rat (Rt) (3)
  4. Canine (Can) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Enzyme Immunoassay (E) (3)
  3. Paraffin Sections (P) (3)
  4. Flow cytometry (F) (1)
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  1. Aff - Purified (3)
  2. Purified (2)
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  1. Rabbit (3)
  2. Goat (2)
Images
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  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (2)
  3. Flow cytometry (F) (1)
  4. Paraffin Sections (P) (1)
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  1. OriGene Technologies (5)
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