CRK antibody
Principal name
CRK antibody
Alternative names for CRK antibody
Proto-oncogene C-crk, p38, Adapter molecule crk
Ncbi ID
9606, NP_005197, NP_005197.3, NM_005206, NP_058431, NP_598417.2, NM_133656, NM_019302, NM_016823
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection))
Background of CRK antibody
The Crk family of adapter proteins including Crk II, Crk I and Crk L consist mostly of SH2 and SH3 domains. Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline- rich motifs, they are involved in a variety of signaling cascades. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor-I (IGFI) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers.
General readings
Muir TW., et al (2006) Biochemistry. 45(29):8874-84.
Inagaki F., et al. (2007) Nat Struct Mol Biol. 14(6):503-10.
Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using CRK antibody (Cat.-No. AP06075PU-N).
Western blot analysis in extracts from Hela cells using CRK antibody (Cat.-No. AP06075PU-N).
Dot blot analysis of CRK (phospho S41) polyclonal antibody ( Cat # PAB0446 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 µg/ml. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.
Dot blot analysis of Phospho-CRK-S41 polyclonal antibody (Cat#AP12756PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
Western Blot analysis of CRK expression in transfected 293T cell line (H00001398-T02) by CRK MaxPab polyclonal antibody.Lane 1: CRK transfected lysate(22.90 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CRK and SHC1 Mahlavu cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CRK and SOS1 HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CRK expression in transfected 293T cell line (H00001398-T02) by CRK MaxPab polyclonal antibody.Lane 1: CRK transfected lysate(22.90 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between FLT1 and CRK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:600 and anti-CRK mouse purified polyclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CRK expression in transfected 293T cell line (H00001398-T02) by CRK MaxPab polyclonal antibody.Lane 1: CRK transfected lysate(22.90 KDa).Lane 2: Non-transfected lysate.
Recombinant human CRK, 1-204aa, His-tagged
Immunohistochemistry (IHC) analyzes of p-Crk2 antibody (Cat.-No.: AP20934PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Immunohistochemistry (IHC) analyzes of Crk2 antibody (Cat.-No.: AP21022PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CRK mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CRK mouse mAb with DAB staining.
Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)
Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Flow cytometric analysis of MCF-7 cells using CRK mouse mAb (blue) and negative control (red).
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK antibody Cat.-No AM06668SU-N with DAB staining.
Immunohistochemical analysis of paraffin-embedded intima cancer tissues using CRK antibody Cat.-No AM06668SU-N with DAB staining.
Immunofluorescence analysis of 3T3-L1 cells using CRK antibody Cat.-No AM06668SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometric analysis of Hela cells using CRK antibody Cat.-No AM06668SU-N (blue) and negative control (red).
Immunohistochemical analysis of CRK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CRK expression in HeLa (A); K562 (B); NIH3T3 (C) whole cell lysates.
Western blot analysis of CrkII expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (CRK Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA325390 | CRK antibody |
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| Rabbit Polyclonal CrkII Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349831 | CRK antibody |
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| Rabbit Polyclonal Anti-CRK Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06075PU-N | CRK antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
$370.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP12756PU-N | CRK pSer41 antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu |
0.4 ml /
$415.00
|
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| OriGene Technologies GmbH | |||||||
| AP20934PU-N | CRK (CRK-II, pTry221) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
0.1 mg /
$370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP21022PU-N | CRK (CRK-II) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
0.1 mg /
$370.00
|
||
| OriGene Technologies GmbH | |||||||