CRHR1 antibody
Principal name
CRHR1 antibody
Alternative names for CRHR1 antibody
CRHR-1, CRFR, CRFR1, CRHR, Corticotropin-releasing factor receptor 1, CRF-R, CRF1, Corticotropin-releasing hormone receptor 1, CRH-R 1
Ncbi ID
9606, NP_001138618.1, NP_004373.2., NP_001138618, NM_004382, NM_001145146, NP_031788.1, NM_007762, NM_030999, NM_001145147, NM_001145148
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of CRHR1 antibody
- Kit Component:
- - KN303823G1, Crhr1 gRNA vector 1 in pCas-Guide vector
- KN303823G2, Crhr1 gRNA vector 2 in pCas-Guide vector
- KN303823D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ungless MA, Singh V, Crowder TL, Yaka R, Ron D, Bonci A. Corticotropin-releasing factor requires CRF binding protein to potentiate NMDA receptors via CRF receptor 2 in dopamine neurons. Neuron. 2003 Jul 31;39(3):401-7. PMID: 12895416
Confocal image ( 3 µg/ml ) of CRHR1 polyclonal antibody ( Cat # PAB7539 ) ( 0.3 µg/ml ) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI ( in blue ) . Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.
CRHR1 polyclonal antibody ( Cat # PAB7539 ) ( 0.1 µg/ml ) staining of human colon lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot (WB) analysis of CRF-RI antibody (Cat.-No.: AP01194PU-N) at 1/500 dilution
Lane1:A549 whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:PC12 whole cell lysate
Lane1:A549 whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:PC12 whole cell lysate
CRFR1 Antibody (Cat. #AP51078PU-N) western blot analysis in K562 cell line lysates (35µg/lane).This demonstrates the CRFR1 antibody detected the CRFR1 protein (arrow).
CRFR1 Antibody (Q103) (Cat. #AP51078PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CRHR1 expression in HeLa (A) whole cell lysates.
TA303282 (0.1µg/ml) staining of human colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Confocal image (3µm) of TA303282 (0.3µg/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.
Western blot analysis of extracts from HT-29 cells, using CRHR1 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF-7 cells, using CRHR1 antibody.The picture on the right is treated with the synthesized peptide.
Anti-CRF1 antibody IHC of human human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of rat hypothalamus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of rat hypothalalmus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human brain, putamen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human brain, cerebellum glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRFR1 / CRHR1 antibody IHC of human brain, glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot analysis of CRHR1 expression in HT-29 cells
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CRHR1 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP16661PU-N | CRHR1 (250-263) antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt, Sh | E, ICC/IF, P |
0.1 mg /
$380.00
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| OriGene Technologies GmbH | |||||||