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CREG1 antibody

Principal name

CREG1 antibody

Alternative names for CREG1 antibody

CREG, Cellular repressor of E1A-stimulated genes 1

SwissProt ID

O75629 (Human), O88668 (Mouse), Q5ZJ73 (Chick)

Gene ID

8804 (CREG1), 433375 (Creg1)

Ncbi ID

9606, NP_003842, ABM85236.1, NP_003842.1, NM_003851, NP_035934.1, NM_011804

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of CREG1 antibody

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. CREG1 antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro.The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.

General readings

Wang,N., et al. (2011) Atherosclerosis 218 (2), 543-551Han,Y.,et al. (2011) J. Mol. Cell. Cardiol. 50 (4), 723-730

Immunoperoxidase of monoclonal antibody to CREG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]

H00008804-M01

CREG1 monoclonal antibody (M01), clone 1B7 Western Blot analysis of CREG1 expression in HL-60 ( Cat # L014V1 ).

H00008804-M01

Detection limit for recombinant GST tagged CREG1 is approximately 0.3ng/ml as a capture antibody.

H00008804-M01

Transfected 293T; WB Suggested Anti-CREG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; CREG1 antibody - N-terminal region (ARP38388_P050) in Transfected 293T cells using Western Blot

ARP38388_P050

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31868PU-N

Western Blot analysis of CREG1 expression in HL-60 using AM31868PU-N CREG1 antibody.

AM31868PU-N

Detection limit for recombinant GST tagged CREG1 is approximately 0.3 ng/ml as a capture antibody.

AM31868PU-N

CREG1 Antibody (N-term) (Cat. #AP51075PU-N) western blot analysis in mouse heart tissue lysates (35µg/lane).This demonstrates the CREG1 antibody detected the CREG1 protein (arrow).

AP51075PU-N

CREG1 Antibody (N-term) (Cat. #AP51075PU-N) western blot analysis in K562 cell line lysates (35µg/lane).This demonstrates the CREG1 antibody detected the CREG1 protein (arrow).

AP51075PU-N

Fig. 1: The tissue extract of mouse lung (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Recombinant Human CREG1 (50ng) protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.