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Collagen type IV antibody

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Principal name

Collagen type IV antibody

Alternative names for Collagen type IV antibody

COL4A1

SwissProt ID

P02462 (Human), P02463 (Mouse), Q7SIB2 (Bovin)

Gene ID

1282 (COL4A1), 12826 (Col4a1)

Ncbi ID

9606, NP_001836, NP_034061, 9913, NP_001836.2, NP_034061.2

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Mam (Mammalia), Por (Porcine), Can (Canine), Fe (Feline), Fi (Fish), Rt (Rat), Orang-Utan, Chk (Chicken)

Available hosts

Mouse, Goat, Rabbit

Available applications

Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Dot blot (Dot), Flow Cytometry (F)

Selected product citations ‐ of R1041

1. Sasse P, Malan D, Fleischmann M, Roell W, Gustafsson E, Bostani T, et al. Perlecan is critical for heart stability. Cardiovasc Res. 2008 Dec 1;80(3):435-44. doi: 10.1093/cvr/cvn225. Epub 2008 Aug 10. PubMed PMID: 18694874.
2. Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem. 2012 May 25;287(22):18700-9. doi: 10.1074/jbc.M111.336073. Epub 2012 Apr 9. PubMed PMID: 22493504.
3. Rodriguez-Teja M, Gronau JH, Breit C, Zhang YZ, Minamidate A, Caley MP, et al. AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival. J Pathol. 2015 Mar;235(4):581-92. doi: 10.1002/path.4485. Epub 2014 Dec 24. PubMed PMID: 25408555. 
4. Rodriguez-Teja, M;Breit, C;Clarke, M;Talar, K;Wang, K;Mohammad, MA;Pickwell, S;Etchandy, G;Stasiuk, GJ;Sturge, J;2016How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases. J Vis Exp  115. PubMed PMID: 27684203.

Background of Collagen type IV antibody

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

AF5910 - Collagen type IV
Staining of FFPE Human skin (20x and 40x) with Collagen type IV Antibody Cat.-No AF5910 (Clone IV-4H12) at 2 µg/ml. Antibody positive in fibillar collagen and in epithelial cells of basement membrane.
AF5910 - Collagen type IV
Staining of FFPE Human epithelial basocellular tumor (20x and 40x) with Collagen type IV Antibody Cat.-No AF5910 (Clone IV-4H12) at 2 µg/ml. Antibody positive in interstitial collagene and in epithelial neoplastic cells of basement membrane.
AM20613PU-N - Collagen type IV
Immunohistochemistry on Human mammary cancer
AP06069PU-N - Collagen type IV
Immunohistochemistry analyzes of Collagen IV antibody Cat-No AP06069PU-N in paraffin-embedded human breast carcinoma tissue.
AP06069PU-N - Collagen type IV
Western blot (WB) analysis of COL4A1 antibody (Cat.-No.: AP06069PU-N) at 1/500 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate.
AP08940PU-N - Collagen type IV
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
BM4067 - Collagen type IV
Swine Kidney, Paraffin Section stained with type IV Collagen Antibody (Clone CIV22) Cat.-No BM4067
BM4067 - Collagen type IV
Human Tonsil, Paraffin Section stained with type IV Collagen Antibody (Clone CIV22) Cat.-No BM4067
C0157-1 - Collagen type IV
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen IV Antibody. The picture on the right is treated with the synthesized peptide.
C0157-1 - Collagen type IV
Western blot analysis of extracts from HeLa cells, using Collagen IV Antibody. The lane on the right is treated with the synthesized peptide.
R1041 - Collagen type IV
R1041 Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
R1041B - Collagen type IV
R1041B Biotin conjugated Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids.
Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen IV is extracellular. Staining: Collagen IV as precipitated red signal with hematoxylin purple nuclear counterstain.
AM33006PU-N - Collagen type IV
Immunohistochemistry on paraffin section of human skin
AM33006PU-N - Collagen type IV
Immunohistochemistry on frozen section of human colon
CPA1254-100ul - Collagen type IV
Immunohistochemical analysis of Collagen 4 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1254-100ul - Collagen type IV
Western blot analysis of Collagen 4 alpha 1 expression in HEK293T (A); mouse endothelial (B) whole cell lysates.
TA311650 - Collagen type IV
Western blot analysis of extracts from HeLa cells, using Collagen IV antibody.The lane on the right is treated with the synthesized peptide.
TA311650 - Collagen type IV
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Collagen IV antibody.The picture on the right is treated with the synthesized peptide.
TA323107 - Collagen type IV
Predicted cell location: Cytoplasm;Basement membrane;Extracellular space;.Extracellular matrix. Positive control: Human lung and breast cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA323107 - Collagen type IV
Predicted cell location: Cytoplasm;Basement membrane;Extracellular space;.Extracellular matrix. Positive control: Human lung and breast cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA323108 - Collagen type IV
Predicted cell location: Cytoplasm;Basement membrane;. Extracellular space;Extracellular matrix. Positive control: Human ovarian and breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA323108 - Collagen type IV
Predicted cell location: Cytoplasm;Basement membrane;. Extracellular space;Extracellular matrix. Positive control: Human ovarian and breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA347635 - Collagen type IV
Western blot analysis on HeLa cell lysate using Collagen IV Antibody

4 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Collagen type IV antibody
R1041 Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit Aff - Purified Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / $515.00
  OriGene Technologies GmbH
 

Top Selling product!

Collagen type IV antibody
Immunohistochemistry analyzes of Collagen IV antibody Cat-No AP06069PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / $370.00
  OriGene Technologies GmbH
+1 additional image

Collagen type IV antibody
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hu E, ICC/IF, IP, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

Collagen type IV antibody
R1041 Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit Aff - Purified Bov, Hu, Mam C, E, IP, P, WB
0.5 mg / $980.00
  OriGene Technologies GmbH

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  1. Primary Antibody (4)
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  1. Collagen type IV (4)
Reactivity
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  2. Bovine (Bov) (3)
  3. Mammalia (Mam) (2)
  4. Mouse (Ms) (1)
Applications
  1. Enzyme Immunoassay (E) (4)
  2. Paraffin Sections (P) (4)
  3. Western blot / Immunoblot (WB) (4)
  4. Immunoprecipitation (IP) (3)
  5. Frozen sections (C) (2)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
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  1. Rabbit (4)
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  2. Western blot / Immunoblot (WB) (1)
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