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CNBP antibody

Principal name

CNBP antibody

Alternative names for CNBP antibody

RNF163, ZNF9, Cellular nucleic acid-binding protein, Zinc finger protein 9

SwissProt ID

O42395 (Chick), P53996 (Mouse), P62633 (Human), P62634 (Rat), Q3T0Q6 (Bovin)

Gene ID

7555 (CNBP), 12785 (Cnbp), 64530

Ncbi ID

NP_003409, 9606, NP_001120668, NP_001120668.1, NM_001127192, NM_001127196, NM_013493, NM_001127193, NM_003418, NM_001127194, NM_001127195, NM_022598

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Bov (Bovine), Chk (Chicken), Por (Porcine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CNBP antibody

CNBP is a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

General readings

McGrath CF, Buckman JS, et al. (2003). J Virol. 77(15):8524-31.

Human brain; WB Suggested Anti-CNBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Human brain; CNBP antibody - N-terminal region (ARP34815_P050) in Human brain cells using Western Blot

ARP34815_P050

human primary myoblasts; CNBP antibody - N-terminal region (ARP34815_P050) validated by WB using human primary myoblasts at 1:1000.; CNBP antibody - N-terminal region (ARP34815_P050) in human primary myoblasts cells using Western Blot

ARP34815_P050

CNBP polyclonal antibody ( Cat # PAB7015 ) ( 2 µg/ml ) staining of human muscle lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7015

The cell lysate of 293T (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CNBP antibody (1:250 - 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot analysis of ZNF9 expression in MCF7 (A); NIH3T3 (B); Raw264.7 (C) whole cell lysates.

TP314473

TP325167

TA303103 (2µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303103

CNBP Antibody (Center) (Cat. #TA324700) western blot analysis in MCF-7 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the CNBP antibody detected the CNBP protein (arrow).