CISD1 antibody
Principal name
CISD1 antibody
Alternative names for CISD1 antibody
C10orf70, ZCD1, MDS029, MitoNEET, CDGSH iron sulfur domain-containing protein 1
Ncbi ID
NP_060934, NP_060934.1, NP_598768.1, NM_134007, NM_001106385, NM_018464
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Flow Cytometry (F), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of CISD1 antibody
- Kit Component:
- - KN303359G1, Cisd1 gRNA vector 1 in pCas-Guide vector
- KN303359G2, Cisd1 gRNA vector 2 in pCas-Guide vector
- KN303359D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Zuris JA., et al. (2011). Proc Natl Acad Sci U S A. 9;108(32):13047-52. Conlan AR., et al. (2011). Acta Crystallogr D Biol Crystallogr. 67(Pt 6):516-23.
Western Blot: The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody.
Anti-CISD1 mouse monoclonal antibody (TA500904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1(RC203308).
Immunofluorescent staining of HeLa cells using anti-CISD1 mouse monoclonal antibody (TA500904).
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-CISD1 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
Flow cytometric Analysis of Jurkat cells, using anti-CISD1 antibody(TA500904),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500905, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500905, Dilution 1:50)
Anti-CISD1 mouse monoclonal antibody (TA500905) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1(RC203308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CISD1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500927, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500927, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CISD1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500927, Dilution 1:50)
Anti-CISD1 mouse monoclonal antibody (TA500927) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1(RC203308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
WB Suggested Anti-CISD1 Antibody; Titration: 1.0 ug/ml; Positive Control: HepG2 Whole Cell
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500904 | CISD1 antibody |
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| CISD1 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3) | |||||||
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Mouse | IgG1 | OTI2B3 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
$379.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Top Selling product! Highly validated antibody!
|
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| TA500905 | CISD1 antibody |
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| anti-CISD1 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1) | |||||||
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Mouse | IgG1 | OTI3A1 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA500927 | CISD1 antibody |
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| anti-CISD1 mouse monoclonal antibody, clone OTI4G3 (formerly 4G3) | |||||||
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Mouse | IgG1 | OTI4G3 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| AM50028PU-N | CISD1 antibody |
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Mouse | IgG1 | AT1A8 | Purified | Hu | E, F, WB |
0.1 ml /
$330.00
|
| OriGene Technologies GmbH | |||||||
| AM50028PU-S | CISD1 antibody |
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Mouse | IgG1 | AT1A8 | Purified | Hu | E, F, WB |
50 µl /
$220.00
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN303359 | Cisd1 - mouse gene knockout kit via CRISPR |
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Mouse | GFP-Puro |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303359BN | Cisd1 - mouse gene knockout kit via CRISPR |
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Mouse | mBFP-Neo |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303359LP | Cisd1 - mouse gene knockout kit via CRISPR |
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Mouse | Luciferase-Puro |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303359RB | Cisd1 - mouse gene knockout kit via CRISPR |
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|
Mouse | RFP-BSD |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303308 | CISD1 |
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| Recombinant protein of human CDGSH iron sulfur domain 1 (CISD1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200391L2V | Lenti ORF particles, Cisd1 (GFP-tagged) - Mouse CDGSH iron sulfur domain 1 (Cisd1), 200 uL, >10^7 TU/mL |
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200 µl /
$930.00
|
|
| OriGene Technologies, Inc. | ||
| MR200391L1V | Lenti ORF particles, Cisd1 (Myc-DDK-tagged) - Mouse CDGSH iron sulfur domain 1 (Cisd1), 200 uL, >10^7 TU/mL |
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|
200 µl /
$830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203338L2V | Lenti ORF particles, Cisd1 (GFP-tagged ORF) - Rat CDGSH iron sulfur domain 1 (Cisd1), 200 uL, >10^7 TU/mL |
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|
200 µl /
$930.00
|
|
| OriGene Technologies, Inc. | ||
| RR203338L1V | Lenti ORF particles, Cisd1 (Myc-DDK-tagged ORF) - Rat CDGSH iron sulfur domain 1 (Cisd1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$830.00
|
|
| OriGene Technologies, Inc. | ||