CD23 antibody
Principal name
CD23 antibody
Alternative names for CD23 antibody
Fc-epsilon-RII, FCER2, CD23A, FCE2, IGEBF, Low affinity immunoglobulin epsilon Fc receptor, Lymphocyte IgE receptor, Immunoglobulin E-binding factor
Ncbi ID
9606, NP_038545.1, NP_001993, NP_001193948, NP_001193948.2, NM_002002, NM_013517
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CD23 antibody
CD23, also known as Fc epsilon RII, or FcεRII, is the "low affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites and is important in regulation of IgE levels. Human rIL-4 is able to induce the expression of low-affinity receptors for IgE (Fc epsilon RL/CD23) on resting B lymphocytes, as determined by the binding of either the anti Fc epsilon RL/CD23-specific mAb 25 or IgE. Stimulation of B cells with insolubilized anti-IgM antibody increases the number of cells expressing Fc epsilon RL/CD23 upon culturing with IL-4 and enhances the level of Fc epsilon RL/CD23 expression on these cells. Fc epsilon RL/CD23 induction is specific for IL-4 since IL-1 alpha, IL-2, IFN-gamma, B cell-derived B cell growth factor (BCGF), and a low-molecular-weight BCGF were ineffective. IFN-gamma strongly inhibited the induction of Fc epsilon RL/CD23 by IL-4. CD23 is a 45 kDa transmembrane glycoprotein associated with MHC Class II antigen, which belongs to the C-type lectin family. Expression of CD23 by B lymphocytes is up-regulated following mitogen or antigen activation. Soluble forms of CD23 are generated by proteolytic cleavage of the membrane molecule. (Defrance T. et al., J Exp Med, 1987,165, 1459 ; Bonnefoy J-Y., et al., 1996, Eur.Respir.J., 9, 635-665).
General readings
Yamamoto, H. et al., J Immunol.,1998; 161(2): 971-7.
Staining of human peripheral blood lymphocytes probed with MOUSE ANTI HUMAN CD23:RPE (SM1788R).
Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE)
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD23 (SM1788PT).
Staining of human peripheral blood lymphocytes probed with MOUSE ANTI HUMAN CD23:RPE (SM1788RT).
Western Blot analysis of FCER2 expression in transfected 293T cell line by FCER2 monoclonal antibody (M14), clone 2A7.Lane 1: FCER2 transfected lysate(36.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged FCER2 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of FCER2 expression in transfected 293T cell line (H00002208-T02) by FCER2 MaxPab polyclonal antibody.Lane 1: FCER2 transfected lysate(35.31 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of FCER2 expression in transfected 293T cell line (H00002208-T02) by FCER2 MaxPab polyclonal antibody.Lane 1: FCER2 transfected lysate(36.50 KDa).Lane 2: Non-transfected lysate.
Human tonsil stained with anti-CD23 antibody
AM11101PU CD23 antibody staining of Human tonsil.
Western Blot analysis of Raji cell lysate with CD23 antibody
Flow Cytometric analysis of Rabbit anti-CD23 antibody in Jurkats (green) compare to negative control of Rabbit IgG (blue).
Immunohistochemical staining of FCER2 on formalin fixed, paraffin embedded human tonsil with FCER2 polyclonal antibody.
Human Tonsil (formalin-fixed, paraffin-embedded) stained with FCER2 antibody AP22699PU-N at 15 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
IHC Image of AM20565PU-NHuman Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Image of AM20565PU-NHuman Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Flow Cytometry Image of AM20565PU-NStaining of normal human peripheral blood cells with 0.5 µg of Purified Mouse IgG1, K isotype control (open histogram) or 0.25 µg of Purified EBVCS2 (colored histogram) followed by FITC Anti-Mouse IgG. Cells in the lymphocyte gate were used for analysis.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD23 antibody (DB 135) shows strong and distinct staining of the follicular dendritic cells in the germinal centres, and a strong and distinct membranous staining of the activated B-cells in the mantle zone of germinal center of the Human tonsil. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD23 antibody (DB 135) shows strong and distinct staining of the follicular dendritic cells in the germinal centres, and a strong and distinct membranous staining of the activated B-cells in the mantle zone of germinal center of the Human tonsil. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD23 antibody (DB 135) shows strong and distinct staining of the follicular dendritic cells in the germinal centres, and a strong and distinct membranous staining of the activated B-cells in the mantle zone of germinal center of the Human tonsil. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD23 antibody (DB 135) shows strong and distinct staining of the follicular dendritic cells in the germinal centres, and a strong and distinct membranous staining of the activated B-cells in the mantle zone of germinal center of the Human tonsil. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
A typical staining pattern with the B-G6 monoclonal antibody of lymphocytes
A typical staining pattern with the B-G6 monoclonal antibody of lymphocytes
A typical staining pattern with the B-G6 monoclonal antibody of lymphocytes
A typical staining pattern with the B-G6 monoclonal antibody of lymphocytes
Human tonsil stained with anti-CD23 Antibody Cat.-No AM32444PU (Clone SP163).
Western Blot analysis of Raji cell lysate with CD23 Antibody Cat.-No AM32444PU (Clone SP163).
Flow Cytometric analysis of Rabbit anti-CD23 Antibody Cat.-No AM32444PU (Clone SP163) in Raji (green) compare to negative control of Rabbit IgG (blue).
Representative Standard Curve
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD23 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD23 Antibody; followed by anti-mouse IgG PE.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801531)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801554)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801571)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2.
Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25ug of Anti-Human CD23 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Cells in the lymphocyte gate were used for analysis.
Western blot analysis of extracts of 293T cell lines, using FCER2 antibody.
Immunohistochemistry staining of Paraffin Tonsil tissue by CD23 antibody (dilution: 1:10 - 1:50; visualization of staining: Membranous)
Immunohistochemistry staining of Paraffin Tonsil, lymph node, chronic lymphocytic leukemia, small lymphocytic lymphoma tissue by CD23 antibody (dilution: 1:100 - 1:500; visualization of staining: Membranous)
Western blot analysis of CD23 expression in Hela (A); mouse brain (B) whole cell lysates.
Immunohistochemical analysis of CD23 staining in human tonsil cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CD57BL/6 mouse splenocytes were stained with purified CD23 (clone B3B4) (filled histogram), or purified rat IgG2a, kappa; (open histogram), followed by anti-rat IgG FITC.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327194 | CD23 antibody |
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| FCER2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
$335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP07157PU-N | CD23 (48-248) antibody |
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 |
Rabbit | Aff - Purified | Hu | E, IP, P, WB |
50 µg /
$480.00
|
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| OriGene Technologies GmbH | |||||||
| DRM013 | CD23 antibody |
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 |
Rabbit | IgG | SP23 | Supernatant | Hu | P |
0.5 ml /
$940.00
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| OriGene Technologies GmbH | |||||||