CAMK2B antibody
Principal name
CAMK2B antibody
Alternative names for CAMK2B antibody
CAM2, CAMKB, CaM-kinase II beta chain, CaM kinase II subunit beta, CaMK-II subunit beta, CAMKIIb, Calcium/calmodulin-dependent protein kinase type II beta chain
Ncbi ID
9606, NP_001211.3, NP_001211, NP_742078.1, NM_172079, NM_172080, NM_172082, NM_172083, NM_172084, NP_742078, NP_001035815.1, NM_007595, NM_001174054, NM_021739, NM_001042354, NM_001042356, NM_172081, NM_001220
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of CAMK2B antibody
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10–40-fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.
Immunoperoxidase of monoclonal antibody to CAMK2B on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. [antibody concentration 3 ug/ml]
CAMK2B monoclonal antibody (M04), clone S11 Western Blot analysis of CAMK2B expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CAMK2B is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CAMK2B is approximately 1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to CAMK2B on HeLa cell. [antibody concentration 25 ug/ml]
CAMK2B monoclonal antibody (M06), clone 6D6 Western Blot analysis of CAMK2B expression in PC-12 ( Cat # L012V1 ).
CAMK2B monoclonal antibody (M06), clone 6D6. Western Blot analysis of CAMK2B expression in HeLa(Cat # L013V1 ).
Western Blot analysis of CAMK2B expression in transfected 293T cell line (H00000816-T01) by CAMK2B MaxPab polyclonal antibody.Lane 1: CAMK2B transfected lysate(55.33 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of CaMKIIβ/γ antibody (Cat.-No.: AP06726PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:Rat colon tissue lysate
Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:Rat colon tissue lysate
Immunoprecipitation of CAMK2B transfected lysate using anti-CAMK2B MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CAMK2B MaxPab mouse polyclonal antibody (B01) (H00000816-B01).
Immunohistochemistry (IHC) analyzes of CaMKIIbeta/gamma/delta antibody (Cat.-No.: AP21111PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of CaMKIIbeta/gamma/delta antibody (Cat.-No.: AP21111PU-N) in extracts from rat brain cells.
IHC Image of AM20579PU-NHuman Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Image of AM20579PU-NHuman Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Image of AM20579PU-NHuman Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CAMK2B monoclonal antibody (AM20579PU-N), clone S11 Western Blot analysis of CAMK2B expression in HeLa NE.
Detection limit for recombinant GST tagged CAMK2B is approximately 0.3 ng/ml as a capture antibody (AM20579PU-N).
Human Fetal heart; WB Suggested Anti-CAMK2B Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Heart; CAMK2B antibody - C-terminal region (ARP61268_P050) in Human Fetal heart cells using Western Blot
Human THP-1; WB Suggested Anti-CAMK2B Antibody. . Titration: 1.0 ug/ml. . Positive Control: THP-1 Whole Cell; CAMK2B antibody - middle region (ARP61269_P050) in Human THP-1 cells using Western Blot
Western blot analysis of CaMK2 beta expression in HeLa (A); mouse brain (B); rat brain (C); PC12 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CAMK2B mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808345)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CAMK2B mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808345)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CAMK2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808345)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CAMK2B mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808345)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CAMK2B mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808345)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CAMK2B mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808345)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMK2B (RC215639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMK2B.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMK2B (RC215639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMK2B.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMK2B (RC215639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMK2B.(1:2000)
Immunohistochemical analysis of CaMK2 beta/gamma (pT287) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CaMK2 beta/gamma (pT287) expression in Raji (A); mouse brain (B); rat brain (C) whole cell lysates.
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/10-25 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CAMK2B antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CAMK2B antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x100)
Western blot analysis of CaMK2-beta/gamma/deltaexpression in Rat braintissue lysates,The lane on the left is treated with the antigen-specific peptide.
Host: Rabbit; Target Name: Camk2b; Sample Tissue: Rat Thymus lysates; Antibody Dilution: 1.0 ug/ml
Western Blot: CaMKII beta Antibody [TA336581] - Analysis of CAMKII beta in A) human, B) mouse and C) rat brain lysate using CAMKII beta antibody at 1:2000.
Western Blot: CaMKII beta Antibody [TA336581] - Analysis of CAMKIIb in rat brain lysate using TA336581 at 1:2000.
Immunocytochemistry/Immunofluorescence: CaMKII beta Antibody [TA336581] - Staining of CAMKIIb using TA336581 in rat hippocampal neurons at a dilution of 1:100, note that staining is rather weak.
WB Suggested Anti-CAMK2B Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart
Western blot analysis of CaMK2- beta/ gamma/ delta phosphorylation expression in Rat braintissue lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from rat brain cells, using CaMK2-ß/?/d (Phospho-Thr287) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-ß/?/d (Phospho-Thr287) antibody.The picture on the right is treated with the synthesized peptide.
