Calpain-2 antibody
Principal name
Calpain-2 antibody
Alternative names for Calpain-2 antibody
Calpain M-type, M-calpain, CAPN2, CANPL2, CANP2, Calpain-2 catalytic subunit, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, Millimolar-calpain, Calpain large polypeptide L2, Calcium-activated neutral proteinase 2
Ncbi ID
NP_001139540.1, 9606, NP_001739, NP_033924.2, NM_009794, NM_017116, NM_001748
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E)
Background of Calpain-2 antibody
- Kit Component:
- - KN302508G1, Capn2 gRNA vector 1 in pCas-Guide vector
- KN302508G2, Capn2 gRNA vector 2 in pCas-Guide vector
- KN302508D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504280)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504280)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504280)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504280)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (RC205642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (RC205642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.
HEK293T cells transfected with either RC205642 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAPN2 antibody(TA504345), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).
Predicted band size: 80kDa. Positive control: Mouse brain and skin tissue, 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: 293T cell lysate Lane3: Mouse skin tissue lysate Lysates: 30 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 40 seconds)
Predicted band size: 80kDa. Positive control: 293T cell and Mouse skin tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Mouse skin tissue lysate Lysates: 30 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 50 seconds)
- Quick-Filters:
- Cited (1)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504280 | Calpain-2 antibody |
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| CAPN2 (Calpain 2) mouse monoclonal antibody, clone OTI3G1 (formerly 3G1) | |||||||
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Mouse | IgG2a | OTI3G1 | Purified | Can, Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
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| TA504345 | Calpain-2 antibody |
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| CAPN2 (Calpain 2) mouse monoclonal antibody, clone OTI1F4 (formerly 1F4) | |||||||
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Mouse | IgG2a | OTI1F4 | Purified | Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
$379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA323973 | Calpain-2 antibody |
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| Anti-CAPN2 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
$335.00
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|
| OriGene Technologies, Inc. | |||||||
| TA323974 | Calpain-2 antibody |
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| Anti-CAPN2 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP00128PU-N | Calpain-2 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 ml /
$395.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305642 | Calpain-2 |
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| Recombinant protein of human calpain 2, (m/II) large subunit (CAPN2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Published recently! |
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Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210127L2V | Lenti ORF particles, Capn2 (GFP-tagged) - Mouse calpain 2 (Capn2), 200 uL, >10^7 TU/mL |
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200 µl /
$1,460.00
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| OriGene Technologies, Inc. | ||
| MR210127L1V | Lenti ORF particles, Capn2 (Myc-DDK-tagged) - Mouse calpain 2 (Capn2), 200 uL, >10^7 TU/mL |
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200 µl /
$1,320.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201011L2V | Lenti ORF particles, Capn2 (GFP-tagged ORF) - Rat calpain 2 (Capn2), 200 uL, >10^7 TU/mL |
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200 µl /
$1,460.00
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|
| OriGene Technologies, Inc. | ||
| RR201011L1V | Lenti ORF particles, Capn2 (Myc-DDK-tagged ORF) - Rat calpain 2 (Capn2), 200 uL, >10^7 TU/mL |
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200 µl /
$1,320.00
|
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| OriGene Technologies, Inc. | ||