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BLK antibody

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Principal name

BLK antibody

Alternative names for BLK antibody

B lymphocyte kinase, p55-BLK, p55BLK, Tyrosine-protein kinase BLK

SwissProt ID

P16277 (Mouse), P51451 (Human)

Gene ID

640 (BLK), 12143 (Blk)

Ncbi ID

NP_001706.2, 9606, NP_001706, BC007371, NM_001715.2, NM_007549, NM_001715

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BLK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

General readings

Dymecki. et al. (1990) Science. 247:332-6.Tatosyan, A.G. et al. (2000) Biochemistry. 65:49-58

AP00057PU-N - BLK
Western blot analysis of BLK expression in mouse kidney tissue lysate.
H00000640-M01 - BLK
Western Blot analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M01), clone 3E5-3A8.Lane 1: BLK transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.
H00000640-M01 - BLK
Detection limit for recombinant GST tagged BLK is approximately 1ng/ml as a capture antibody.
H00000640-M02 - BLK
BLK monoclonal antibody (M02), clone 7A12. Western Blot analysis of BLK expression in PC-12(Cat # L012V1 ).
H00000640-M02 - BLK
BLK monoclonal antibody (M02), clone 7A12. Western Blot analysis of BLK expression in Raw 264.7.
H00000640-M02 - BLK
Detection limit for recombinant GST tagged BLK is approximately 0.03ng/ml as a capture antibody.
PAB3417 - BLK
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BLK polyclonal antibody ( Cat # PAB3417 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB3417 - BLK
Western blot analysis of BLK polyclonal antibody ( Cat # PAB3417 ) transiently transfected HEK293 cell line lysate ( 1 µg/lane ) . BLK polyclonal antibody ( arrow ) was detected using the purified polyclonal antibody ( 1 : 1500 dilution ) .
PAB3417 - BLK
Western blot analysis of BLK polyclonal antibody ( Cat # PAB3417 ) in mouse kidney tissue lysate. BLK ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
H00000640-B01 - BLK
Western Blot analysis of BLK expression in transfected 293T cell line (H00000640-T01) by BLK MaxPab polyclonal antibody.Lane 1: BLK transfected lysate(55.55 KDa).Lane 2: Non-transfected lysate.
H00000640-D01P - BLK
Western Blot analysis of BLK expression in transfected 293T cell line (H00000640-T01) by BLK MaxPab polyclonal antibody.Lane 1: BLK transfected lysate(57.70 KDa).Lane 2: Non-transfected lysate.
ARP46061_T100 - BLK
Human HepG2; WB Suggested Anti-BLK Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; BLK antibody - middle region (ARP46061_T100) in Human HepG2 cells using Western Blot
A8081-1 - BLK
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BLK (Phospho-Tyr501) Antibody. The picture on the right is treated with the synthesized peptide.
AM06135SU-N - BLK
Immunohistochemical analysis of paraffin-embedded human breast tissue (A), lymph tissue (B) and skin carcinoma (C), showing membrane localization using BLK antibody Cat.-No AM06135SU-N with DAB staining.
AM06138SU-N - BLK
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), lymph tissue (B) and skin carcinoma (C), showing membrane localization using BLK antibody Cat.-No AM06138SU-N with DAB staining.
AM06138SU-N - BLK
Western blot analysis using BLK antibody Cat.-No AM06138SU-N against truncated BLK recombinant protein.
AM06549SU-N - BLK
Immunofluorescence analysis of Hela cells using BLK antibody Cat.-No AM06549SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled
AM06549SU-N - BLK
Western blot analysis using BLK antibody Cat.-No AM06549SU-N against HEK293 (1) and BLK (AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate.
AM06549SU-N - BLK
Flow cytometric analysis of HL-60 cells using BLK antibody Cat.-No AM06549SU-N (green) and negative control (purple).
AR50048PU-N - BLK
AP55751PU-S - BLK
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody AP55751PU-N (left)or the same antibody preincubated with blocking peptide (right).
TA300020 - BLK
Western blot analysis of anti-BLK Pab in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
TA300020 - BLK
(TOP) Western blot analysis of anti-BLK Pab transiently transfected HEK-293 cell line lysate (1ug/lane). BLK pab(arrow) was detected using the purified Pab (1:1500 dilution). (BOTTOM) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
TA311214 - BLK
(0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA338995 - BLK
WB Suggested Anti-BLK Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate
TA349715 - BLK
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A172 cells, Primary antibody: (BLK Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
TA349715 - BLK
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (BLK Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
TA349715 - BLK
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (BLK Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
TA313508 - BLK
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody.The picture on the right is treated with the synthesized peptide.

3 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BLK antibody
Immunohistochemical analysis of paraffin-embedded human breast tissue (A), lymph tissue (B) and skin carcinoma (C), showing membrane localization using BLK antibody Cat.-No AM06135SU-N with DAB staining. Mouse IgG2a 9D10D1 Ascites Hu P
0.1 ml / $420.00
  OriGene Technologies GmbH

BLK antibody
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), lymph tissue (B) and skin carcinoma (C), showing membrane localization using BLK antibody Cat.-No AM06138SU-N with DAB staining. Mouse IgG1 9D10B7H6 Ascites Hu E, P, WB
0.1 ml / $340.00
  OriGene Technologies GmbH
+1 additional image

BLK antibody
Immunofluorescence analysis of Hela cells using BLK antibody Cat.-No AM06549SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled Mouse IgG1 1E6 Ascites Hu E, F, ICC/IF, WB
0.1 ml / $380.00
  OriGene Technologies GmbH
+2 additional images

3 Item(s)

per page

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  1. Primary Antibody (3)
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  1. Enzyme Immunoassay (E) (2)
  2. Paraffin Sections (P) (2)
  3. Western blot / Immunoblot (WB) (2)
  4. Flow cytometry (F) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
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  2. 3+ images (1)
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  2. Western blot / Immunoblot (WB) (2)
  3. Flow cytometry (F) (1)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
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