BBOX1 antibody
Principal name
BBOX1 antibody
Alternative names for BBOX1 antibody
Gamma-BBH, BBOX, Gamma-butyrobetaine dioxygenase, Gamma-butyrobetaine,2-oxoglutarate dioxygenase, Gamma-butyrobetaine hydroxylase
Ncbi ID
9606, NP_003977, NP_003977.1, NP_569719.1, NM_130452, NM_022629, NM_003986
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of BBOX1 antibody
- Kit Component:
- - KN301994G1, Bbox1 gRNA vector 1 in pCas-Guide vector
- KN301994G2, Bbox1 gRNA vector 2 in pCas-Guide vector
- KN301994D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to BBOX1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged BBOX1 is approximately 1ng/ml as a capture antibody.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged BBOX1 is approximately 1 ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505713)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505731)
Anti-BBOX1 mouse monoclonal antibody (TA505731) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1(RC203156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505748)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505752)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505753)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505753)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505753)
Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (TA505753).
Anti-BBOX1 mouse monoclonal antibody (TA505753) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1(RC203156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505786)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505786)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505786)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Anti-BBOX1 mouse monoclonal antibody (TA505787) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1(RC203156).
Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (TA505787).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505788)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505788)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505789)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505789)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505790)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BBOX1 mouse monoclonal antibody (UM500084). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. TA308141 diluted at 1:500.
Sample(30 ug of whole cell lysate). A:MOLT4. 7.5% SDS PAGE. TA308141 diluted at 1:500
BBOX1 antibody detects BBOX1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. BBOX1 antibody (TA308141) dilution: 1:500.
Immunofluorescence analysis of methanol-fixed A431, using BBOX1(TA308141) antibody at 1:500 dilution.
Western blot analysis of extracts of various cell lines, using BBOX1 antibody.
- Quick-Filters:
- Highly Validated (7)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM500084 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody,clone UMAB119 | |||||||
 |
Mouse | IgG1 | UMAB119 | Purified | Hu | P |
0.1 ml /
$468.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
Highly validated antibody!
|
||||||
| UM500085 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody,clone UMAB120 | |||||||
 |
Mouse | IgG1 | UMAB120 | Purified | Hu | P |
0.1 ml /
$468.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
|
||||||
| UM570084 | BBOX1 antibody |
||||||
| BBOX1 mouse monoclonal antibody,clone UMAB119 | |||||||
|
Mouse | IgG1 | UMAB119 | Purified | Hu | P |
30 µl /
$180.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
|
||||||
| UM570085 | BBOX1 antibody |
||||||
| BBOX1 mouse monoclonal antibody,clone UMAB120 | |||||||
|
Mouse | IgG1 | UMAB120 | Purified | Hu | P |
30 µl /
$180.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Highly validated antibody!
|
||||||
| TA308141 | BBOX1 antibody |
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| Rabbit Polyclonal antibody to BBOX1 (butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
$415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA505753 | BBOX1 antibody |
||||||
| BBOX1 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9) | |||||||
 |
Mouse | IgG1 | OTI4F9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
$379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA505786 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI8B12 (formerly 8B12) | |||||||
 |
Mouse | IgG1 | OTI8B12 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA332929 | BBOX1 antibody |
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| Rabbit anti-BBOX1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
$335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA505713 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6) | |||||||
 |
Mouse | IgG1 | OTI1D6 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505731 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) | |||||||
 |
Mouse | IgG1 | OTI1E11 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA505748 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI1D11 (formerly 1D11) | |||||||
 |
Mouse | IgG1 | OTI1D11 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505752 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI3B6 (formerly 3B6) | |||||||
 |
Mouse | IgG1 | OTI3B6 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA505787 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8) | |||||||
 |
Mouse | IgG1 | OTI2C8 | Purified | Hu | ICC/IF, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA505788 | BBOX1 antibody |
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| BBOX1 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1) | |||||||
 |
Mouse | IgG1 | OTI2G1 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA505789 | BBOX1 antibody |
||||||
| BBOX1 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4) | |||||||
 |
Mouse | IgG1 | OTI2F4 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA505790 | BBOX1 antibody |
||||||
| BBOX1 mouse monoclonal antibody, clone OTI8A8 (formerly 8A8) | |||||||
 |
Mouse | IgG1 | OTI8A8 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AM20958PU-N | BBOX1 antibody |
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 |
Mouse | IgG1 | 6H3 | Hu | E, P, WB |
50 µg /
$475.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303156 | BBOX1 |
||||
| Recombinant protein of human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC203156L1V | Lenti ORF particles, BBOX1 (Myc-DDK tagged) - Human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1) , 200 uL, >10^7 TU/mL |
|
 |
200 µl /
$1,000.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206051L2V | Lenti ORF particles, Bbox1 (GFP-tagged) - Mouse butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) (Bbox1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$930.00
|
|
| OriGene Technologies, Inc. | ||