ATG13 antibody
Principal name
ATG13 antibody
Alternative names for ATG13 antibody
KIAA0652, Autophagy-related protein 13
Ncbi ID
9606, NP_001136145, NM_014741, NP_001136145.1, NP_663503.1, NM_001142673
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ATG13 antibody
- Kit Component:
- - KN301729G1, Atg13 gRNA vector 1 in pCas-Guide vector
- KN301729G2, Atg13 gRNA vector 2 in pCas-Guide vector
- KN301729D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hosokawa, N., et al. Autophagy 5(7):973-979(2009)
Mercer, C.A., et al. Autophagy 5(5):649-662(2009)
Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009)
Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009)
Hosokawa, N., et al. Mol. Biol. Cell 20(7):1981-1991(2009)
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma reacted with KIAA0652 Antibody (N-term) Cat.-No AP52338PU-N, which was peroxidase conjugated to the secondary antibody and followed byDAB staining.
Western blot analysis of KIAA0652 Antibody (N-term) Cat.-No AP52338PU-N in MDA-MB435, CEM, 47D cell line and mouse cerebellum tissue lysates (35ug/lane).
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
WB using Anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. primary antibody was used at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000.
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ATG13 in mouse heart with ATG13 antibody at 5 ug/mL.
Immunofluorescence of ATG13 in Mouse Heart tissue with ATG13 antibody at 20 ug/mL.
Predicted band size: 57 kDa . Positive control: Human brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using ATG13 antibody.
Immunohistochemistry of paraffin-embedded mouse lung using ATG13 antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of A549 cell using ATG13 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-ATG13 Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole Cell There is BioGPS gene expression data showing that ATG13 is expressed in HEK293T
Lysate were prepared from HEK293A cell either absent of RISC(Lane 1) or treated with Atg13 siRNA(Lane 2). Immunoblotting was performed using ABC130 at a dilution of 1:500 and an anti-Tubulin antibody (from the researcher)
Lysates were prepared from MEFs either absent of RISC(Lane 1) or treated with Atg13 siRNA(Lane 2). Immunoblotting was performed using Anti-ATG13, polyclonal [ATG13] at a dilution of 1:500 and an anti-Ulk1 antibody(from the researcher).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52338PU-N | ATG13 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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