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ASPH antibody

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Principal name

ASPH antibody

Alternative names for ASPH antibody

ASP beta-hydroxylase, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase, Aspartate beta-hydroxylase, Peptide-aspartate beta-dioxygenase

SwissProt ID

Q12797 (Human), Q28056 (Bovin), Q8BSY0 (Mouse)

Gene ID

444 (ASPH), 65973 (Asph)

Ncbi ID

NP_004309, 9606, NP_064549, NP_001158222, NP_115856.1, NM_004318, NP_115855, NP_115857, NP_001158227, NP_001158222.1, NP_001171321.1, NM_023066, NM_001177850, NM_001177853, NM_001177856, NM_133723, NM_001177849, NM_020164, NM_001164756, NM_001164755, NM_001164752, NM_001164754, NM_001164751

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Eq (Equine), Por (Porcine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of ASPH antibody

This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.

General readings

Kwon SJ., et al. (2009) Biochem Biophys Res Commun. 390(4):1389-94.

ARP34195_T100 - ASPH
Human Jurkat; WB Suggested Anti-ASPH Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ASPH antibody - middle region (ARP34195_T100) in Human Jurkat cells using Western Blot
H00000444-B01 - ASPH
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T01) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
H00000444-B01 - ASPH
ASPH MaxPab polyclonal antibody. Western Blot analysis of ASPH expression in HepG2.
H00000444-M09 - ASPH
Detection limit for recombinant GST tagged ASPH is approximately 1ng/ml as a capture antibody.
H00000444-B01P - ASPH
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T01) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
H00000444-B01P - ASPH
ASPH MaxPab polyclonal antibody. Western Blot analysis of ASPH expression in HepG2.
H00000444-B02P - ASPH
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T02) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(23.1 KDa).Lane 2: Non-transfected lysate.
ARP51357_T100 - ASPH
Human kidney; ASPH antibody - N-terminal region (ARP51357_T100) in Human kidney cells using Immunohistochemistry
ARP51357_T100 - ASPH
Human HepG2; WB Suggested Anti-ASPH Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that ASPH is expressed in HepG2.; ASPH antibody - N-terminal region (ARP51357_T100) in Human HepG2 cells using Western Blot
ARP51356_P050 - ASPH
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. ASPH is supported by BioGPS gene expression data to be expressed in 721_B.; ASPH antibody - N-terminal region (ARP51356_P050) in Human 721_B cells using Western Blot
ARP51356_P050 - ASPH
Human HepG2; WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that ASPH is expressed in HepG2.; ASPH antibody - N-terminal region (ARP51356_P050) in Human HepG2 cells using Western Blot
H00000444-D01P - ASPH
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T04) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
H00000444-D03P - ASPH
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T03) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(23.80 KDa).Lane 2: Non-transfected lysate.
AR09383PU-L - ASPH
AR09383PU-L - ASPH
Recombinant human ASPH, 75-270 aa, His-tagged: 15% SDS-PAGE (3 µg)
H00000444-D03 - ASPH
Immunoprecipitation of ASPH transfected lysate using anti-ASPH MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ASPH purified MaxPab mouse polyclonal antibody (B02P) (H00000444-B02P).
ARP34691_P050 - ASPH
Human Small intestine; Immunohistochemistry with Small intestine, myenteric plexus tissue at an antibody concentration of 5ug/ml using anti-ASPH antibody (ARP34691_P050); ASPH antibody - middle region (ARP34691_P050) in Human Small intestine cells using Immunohistochemistry
ARP34691_P050 - ASPH
Human ACHN; WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; ASPH antibody - middle region (ARP34691_P050) in Human ACHN cells using Western Blot
AP50276PU-N - ASPH
ASPH Antibody (Center) (Cat. #AP50276PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASPH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
AP50276PU-N - ASPH
ASPH Antibody (Center) (Cat. #AP50276PU-N) western blot analysis in A549 cell line lysates (35µg/lane).This demonstrates the ASPH antibody detected the ASPH protein (arrow).
TP311817 - ASPH
TP316796 - ASPH
TP323930 - ASPH
TP328203 - ASPH
TA335749 - ASPH
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: MCF7 cell lysate
TA335750 - ASPH
WB Suggested Anti-ASPH Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
TA337291 - ASPH
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate
TA337291 - ASPH
Immunohistochemistry with Small intestine, myenteric plexus tissue at an antibody concentration of 5 ug/ml using anti-ASPH antibody
TA339206 - ASPH
1Human 721_B; Host: Rabbit; Target Name: ASPH; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/ml ASPH is supported by BioGPS gene expression data to be expressed in 721_B
TA339206 - ASPH
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ASPH is expressed in HepG2
TA339207 - ASPH
WB Suggested Anti-ASPH Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ASPH is expressed in HepG2
TA339207 - ASPH
Human kidney
TA343455 - ASPH
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASPH (Center) antibody
ASPH Antibody (Center) (Cat. #AP50276PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASPH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / $415.00
  OriGene Technologies GmbH
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