ARFGAP3 antibody
Principal name
ARFGAP3 antibody
Alternative names for ARFGAP3 antibody
ARF GAP 3, ARFGAP1, ADP-ribosylation factor GTPase-activating protein 3
Ncbi ID
9606, NM_014570, NP_055385, NP_055385.3, NP_001135765.1, NP_079721.2, NM_025445, NM_001044273, NM_001142293
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of ARFGAP3 antibody
- Kit Component:
- - KN301477G1, Arfgap3 gRNA vector 1 in pCas-Guide vector
- KN301477G2, Arfgap3 gRNA vector 2 in pCas-Guide vector
- KN301477D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Liu X, Zhang C, Xing G, Chen Q, He F.
Functional characterization of novel human ARFGAP3.
FEBS Lett 2001 Feb 9;490(1-2):79-83
PMID: 11172815
ARFGAP3 polyclonal antibody ( Cat # PAB6069 ) staining ( 2 µg/ml ) of mouse brain extracts ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human 721_B; WB Suggested Anti-ARFGAP3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: 721_B Whole Cell. ARFGAP3 is supported by BioGPS gene expression data to be expressed in 721_B.; ARFGAP3 antibody - N-terminal region (ARP61869_P050) in Human 721_B cells using Western Blot
TA302737 staining (2µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts from HeLa cells, using ARFGAP3 antibody.The lane on the right is treated with the synthesized peptide.
Western Blot of Rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 µg per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50kDa.
Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent sinal.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302392 | ARFGAP3 (transcript variant 1) |
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| Recombinant protein of human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||