Apelin Receptor antibody
Principal name
Apelin Receptor antibody
Alternative names for Apelin Receptor antibody
APLNR, AGTRL1, APJ, HG11, G-protein coupled receptor APJ, Angiotensin receptor-like 1
Ncbi ID
NP_112639.1, NP_005152.1, ABM81770.1, NP_005152, NP_035914.1, NM_031349, NM_005161
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of Apelin Receptor antibody
- Kit Component:
- - KN301402G1, Agtrl1 gRNA vector 1 in pCas-Guide vector
- KN301402G2, Agtrl1 gRNA vector 2 in pCas-Guide vector
- KN301402D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot (WB) analysis of APJR-1 antibody (Cat.-No. AP01191PU-N) in extracts from A549 cells.
Western blot (WB) analysis of APJR-1 antibody (Cat.-No. AP01191PU-N) in extracts from 293.
Human MCF-7; WB Suggested Anti-APLNR Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; APLNR antibody - N-terminal region (ARP59985_P050) in Human MCF-7 cells using Western Blot
Western blot analysis of APJ expression in HeLa (A); mouse lung (B); rat liver (C) whole cell lysates.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. TA308705 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Apelin Receptor(TA308705) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed A431, using Apelin Receptor(TA308705) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308706 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Raji. . 10% SDS PAGE. . TA308706 diluted at 1:500.
Apelin Receptor antibody [C2C3], C-term detects APLNR protein at membrane on Gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Gastric carcinoma. Apelin Receptor antibody [C2C3], C-term (TA308706) dilution: 1:500.
Western blot analysis of extracts from 293 cells, using AGTRL1 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF-7 cells, using AGTRL1 antibody.The picture on the right is treated with the synthesized peptide.
Anti-Apelin Receptor antibody IHC of human brain, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Predicted band size: 43 kDa . Positive control: OP9 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using APLNR antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
Anti-Apelin Receptor antibody IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-APLNR/ Apelin Receptor / APJ antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-APLNR/ Apelin Receptor / APJ antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-APLNR/ Apelin Receptor / APJ antibody IHC of human brain vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
WB Suggested Anti-APLNR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (APLNR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (APLNR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN207576 | APLNR - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||