AMCase antibody
Principal name
AMCase antibody
Alternative names for AMCase antibody
Acidic mammalian chitinase, CHIA, TSA1902, Lung-specific protein TSA1902
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of AMCase antibody
- Kit Component:
- - KN206441G1, CHIA gRNA vector 1 in pCas-Guide vector
- KN206441G2, CHIA gRNA vector 2 in pCas-Guide vector
- KN206441D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chou,Y.T., (2006) Biochemistry 45 (14), 4444-4454
CHIA MaxPab polyclonal antibody. Western Blot analysis of CHIA expression in human liver.
Western Blot analysis of CHIA expression in transfected 293T cell line (H00027159-T01) by CHIA MaxPab polyclonal antibody.Lane 1: CHIA transfected lysate(40.48 KDa).Lane 2: Non-transfected lysate.
Human HeLa; WB Suggested Anti-CHIA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; CHIA antibody - middle region (ARP42602_P050) in Human HeLa cells using Western Blot
Transfected 293T; WB Suggested Anti-CHIA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; CHIA antibody - N-terminal region (ARP42601_P050) in Transfected 293T cells using Western Blot
Hum. Fetal Heart; Host: Rabbit . Target Name: CHIA . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; CHIA antibody - N-terminal region (ARP42601_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Liver; Host: Rabbit . Target Name: CHIA . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; CHIA antibody - N-terminal region (ARP42601_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: CHIA . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; CHIA antibody - N-terminal region (ARP42601_P050) in Hum. Fetal Lung cells using Western Blot
Western Blot analysis of CHIA expression in transfected 293T cell line (H00027159-T01) by CHIA MaxPab polyclonal antibody.Lane 1: CHIA transfected lysate(40.10 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of CHIA Antibody (N-term) (Cat. #AP50895PU-N) in mouse liver tissue lysates (35µg/lane). CHIA (arrow) was detected using the purified Pab.
CHIA Antibody (N-term) (Cat.#AP50895PU-N) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-CHIA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate
WB Suggested Anti-CHIA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
Host: Rabbit; Target Name: CHIA; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0ug/ml
Host: Rabbit; Target Name: CHIA; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0ug/ml
Host: Rabbit; Target Name: CHIA; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP50895PU-N | AMCase (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | F, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760630 | AMCase (N-term HIS tag, transcript variant 2) |
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| Purified recombinant protein of Human chitinase, acidic (CHIA), transcript variant 2, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
$205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205616L2V | Lenti ORF particles, Chia (GFP-tagged) - Mouse chitinase, acidic (cDNA clone MGC:18771 IMAGE:4165150), 200 uL, >10^7 TU/mL |
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 |
200 µl /
$1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR207559L2V | Lenti ORF particles, Chia (GFP-tagged) - Mouse chitinase, acidic (Chia), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,150.00
|
|
| OriGene Technologies, Inc. | ||
| MR205616L1V | Lenti ORF particles, Chia (Myc-DDK-tagged) - Mouse chitinase, acidic (cDNA clone MGC:18771 IMAGE:4165150), 200 uL, >10^7 TU/mL |
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|
200 µl /
$1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR207559L1V | Lenti ORF particles, Chia (Myc-DDK-tagged) - Mouse chitinase, acidic (Chia), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,030.00
|
|
| OriGene Technologies, Inc. | ||