ALDH1 antibody
Principal name
ALDH1 antibody
Alternative names for ALDH1 antibody
Retinal dehydrogenase 1, RALDH1, ALDH1A1, ALDC, PUMB1, Aldehyde dehydrogenase cytosolic, ALHDII, ALDH-E1, Aldehyde dehydrogenase family 1 member A1
Ncbi ID
9606, NP_000680, NP_000680.2, NM_000689, NP_038495.2, NM_013467, NM_022407
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ALDH1 antibody
ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005). ALDH1A1 protein is a known mesencephalic dopaminergic marker. ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid .ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea .ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development .The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells.
Detection limit for recombinant GST tagged ALDH1A1 is approximately 0.3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 polyclonal antibody ( Cat # PAB3123 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ALDH1A1 ( arrow ) using rabbit ALDH1A1 polyclonal antibody ( Cat # PAB3123 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the ALDH1A1 gene ( Lane 2 ) ( Origene Technologies ) .
ALDH1A1 polyclonal antibody ( Cat # PAB6018 ) staining ( 0.03 µg/ml ) of human liver lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ALDH1A1 polyclonal antibody ( Cat # PAB6978 ) ( 1 µg/ml ) staining of human liver Lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
ALDH1A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH1A1 expression in human liver.
Western Blot analysis of ALDH1A1 expression in transfected 293T cell line (H00000216-T01) by ALDH1A1 MaxPab polyclonal antibody.Lane 1: ALDH1A1 transfected lysate(55.11 KDa).Lane 2: Non-transfected lysate.
ALDH1A1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH1A1 expression in mouse lung.
Western Blot analysis of ALDH1A1 expression in transfected 293T cell line (H00000216-T01) by ALDH1A1 MaxPab polyclonal antibody.Lane 1: ALDH1A1 transfected lysate(54.90 KDa).Lane 2: Non-transfected lysate.
ALDH1A1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH1A1 expression in HepG2.
Recombinant human Aldehyde dehydrogenase 1A1, 1-501 aa: 15% SDS-PAGE (3 µg)
Formalin-Fixed Paraffin-Embedded Human Testis stained with ALDH1A1 antibody Cat.-No AP08840PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western Blot analysis of Human Liver Lysate (35 µg protein in RIPA buffer) using ALDH1A1 antibody Cat.-No AP08840PU-N. Primary incubation was 1 hour. Detected by chemiluminescence.
ALDH1A1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH1A1 expression in HepG2.
ALDH1A1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH1A1 expression in mouse lung.
Immunoprecipitation of ALDH1A1 transfected lysate using anti-ALDH1A1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ALDH1A1 MaxPab mouse polyclonal antibody (B01) (H00000216-B01).
Human Cornea; . Sample Type: Human Cornea. Dilution:. 1:200; ALDH1A1 antibody - middle region (ARP58737_P050) in Human Cornea cells using Immunohistochemistry
Human ACHN; WB Suggested Anti-ALDH1A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate. ALDH1A1 is supported by BioGPS gene expression data to be expressed in ACHN.; ALDH1A1 antibody - middle region (ARP58737_P050) in Human ACHN cells using Western Blot
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of ALDH1A1 expression in transfected 293T cell line. Lane 1: ALDH1A1 transfected lysate (Predicted MW: 54.9 KDa). Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ALDH1A1 is 1 ng/ml as a capture antibody.
Immunoprecipitation of ALDH1A1 transfected lysate using anti-ALDH1A1 monoclonal antibody and Protein A Magnetic Bead.
Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and lung cancer (right) using ALDH1A1 antibody Cat.-No AM06513SU-N with DAB staining.
Western blot analysis using ALDH1A1 antibody Cat.-No AM06513SU-N against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.
Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# TA302024). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody (Center)(Cat.#TA302024), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence analysis of anti-ALDH1A1 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow cytometric analysis of HepG2 cells using ALDH1A1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA302409 (1µg/ml) staining of Human Liver Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ALDH1A1 and probed with TA302409 (mock transfection in first lane).
TA302641 staining (0.03µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Predicted band size: 57 kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/450 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human pancreatic cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human pancreatic cancer tissue using ALDH1A1/ALDH1A2/ALDH1A3 antibody at dilution 1/45, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 57 kDa. Positive control: Mouse liver tissue lysate . Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ALDH1A1/ALDH1A2/ALDH1A3 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification: x200)
ALDH1A1 Monoclonal Antibody(Cat. #TA324334) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).
ALDH1A1 Monoclonal Antibody (Cat. #TA324334) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were incubated with TA324334 ALDH1A1 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat#TA324334) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysates from HepG2 cell line and human lung tissue lysate (from left to right), using ALDH1A1 Antibody(Cat. #TA324559). TA324559 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
ALDH1A1 Antibody (Cat. #TA324559) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were incubated with TA324559 ALDH1A1 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
ALDH1A1 Antibody (Cat. #TA324559) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using ALDH1A1 antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of A549 cell using ALDH1A1 antibody. Blue: DAPI for nuclear staining.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302641 | ALDH1 (C-term) antibody |
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| Goat Polyclonal Antibody against ALDH1A1 (C Terminus) | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
$360.00
|
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| OriGene Technologies, Inc. | |||||||
Published recently! |
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| AP08840PU-N | ALDH1 (476-488) antibody |
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Goat | IgG | Aff - Purified | Bov, Hu, Sh | E, P, WB |
50 µg /
$425.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
Published recently! |
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| AM31717PU-N | ALDH1 (1-502) antibody |
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Mouse | IgG2a | 1G6 | Purified | Hu | E, IP, P, WB |
50 µg /
$475.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA321182 | ALDH1 antibody |
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| Anti-ALDH1A1/ALDH1A2/ALDH1A3 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321183 | ALDH1 antibody |
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| Anti-ALDH1A1/ALDH1A2/ALDH1A3 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM06513SU-N | ALDH1 antibody |
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Mouse | IgG1 | 5A11 | Ascites | Hu | E, P, WB |
0.1 ml /
$380.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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