AGR2 antibody
Principal name
AGR2 antibody
Alternative names for AGR2 antibody
Anterior gradient protein 2 homolog, AG2, AG-2, HPC8, Secreted cement gland protein XAG-2 homolog
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of AGR2 antibody
Origilly discovered in Xenopus laevis wherein it plays a role during early neural development, AGR2 (anterior gradient protein 2 homolog) is now known as a member of protein disulfide isomerase (PDI) family of ER-resident proteins which is expressed in a variety of normal as well as transformed epithelial tissues. AGR2 has been implicated in the folding of proteins as a chaperone protein. It interacts with LYPD3, DAG1 (alphaDAG1) and MUC2 (disulfide-linked), and is essential for MUC2 translation as well as secretion. AGR2 plays a key role in the production of mucus by intestil cells and has also been suggested to be a proto-oncogene that may involve in cellular migration, differentiation and growth. Differential expression of AGR2 was first found in estrogen receptor (ER)-positive breast cancer cell lines and subsequently, elevated AGR2 levels have been seen in subsets of other cancers also wherein its upregulation enhances classical hallmarks of cancer such as metastasis, invasion, colony formation, and proliferation.
General readings
Innes HE, Liu D, Barraclough R, Davies MP, O'Neill PA, Platt-Higgins A, de Silva Rudland S, Sibson DR, Rudland PS.
Significance of the metastasis-inducing protein AGR2 for outcome in hormonally treated breast cancer patients.
Br J Cancer. 2006 Apr 10;94(7):1057-65.
PMID: 16598187
Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon (formalin-fixed, paraffin-embedded) stained with AGR2 antibody AP22561PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with AGR2 antibody followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.01 µg/ml) staining of Human Duodenum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
15% SDS-PAGE (3 µg)
Human Colon; Colon; AGR2 antibody - middle region (ARP42290_P050) in Human Colon cells using Immunohistochemistry
Fetal Muscle; Host: Rabbit. Target Name:AGR2. Sample Tissue:Fetal Muscle. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane. Gel Concentration: 0.12%.
AGR2 monoclonal antibody (M01), clone 1E5. Western Blot analysis of AGR2 expression in human colon.
Western Blot analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M01), clone 1E5.Lane 1: AGR2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
AGR2 monoclonal antibody (M01), clone 1E5. Western Blot analysis of AGR2 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged AGR2 is approximately 3ng/ml as a capture antibody.
AGR2 monoclonal antibody (M01), clone 1E5. Western Blot analysis of AGR2 expression in human colon.
AGR2 monoclonal antibody (M01), clone 1E5. Western Blot analysis of AGR2 expression in MCF-7 ( Cat # L046V1 ).
AGR2 monoclonal antibody (M02), clone 3G1. Western Blot analysis of AGR2 expression in MCF-7 ( Cat # L046V1 ).
Western Blot analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M02), clone 3G1.Lane 1: AGR2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged AGR2 is approximately 3ng/ml as a capture antibody.
AGR2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of AGR2 expression in MCF-7 ( Cat # L046V1 ).
Western Blot analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M03), clone 1C3.Lane 1: AGR2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged AGR2 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M05), clone 1C9.Lane 1: AGR2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
AGR2 monoclonal antibody (M05), clone 1C9. Western Blot analysis of AGR2 expression in human colon.
Detection limit for recombinant GST tagged AGR2 is approximately 1ng/ml as a capture antibody.
AGR2 monoclonal antibody (M05), clone 1C9. Western Blot analysis of AGR2 expression in human colon.
Western Blot analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M06), clone 6H2.Lane 1: AGR2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged AGR2 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M07), clone 7E8.Lane 1: AGR2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged AGR2 is approximately 1ng/ml as a capture antibody.
Detection of Agr2 in mouse prostate using Agr2 polyclonal antibody ( Cat # PAB12150 ).
Detection of Agr2 in mouse kidney using Agr2 polyclonal antibody ( Cat # PAB12150 ).
Detection of AGR2 on mouse kidney using AGR2 polyclonal antibody ( Cat # PAB12491 ).
Detection of AGR2 on mouse prostate using AGR2 polyclonal antibody ( Cat # PAB12491 ).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 polyclonal antibody ( Cat # PAB4864 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of AGR2 ( arrow ) using rabbit AGR2 polyclonal antibody ( Cat # PAB4864 ) . 293 cell lysate ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the AGR2 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of AGR2 polyclonal antibody ( Cat # PAB4864 ) in T-47D cell lysate ( 35 µg/lane ) . AGR2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 polyclonal antibody ( Cat # PAB4865 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of AGR2 ( arrow ) using rabbit AGR2 polyclonal antibody ( Cat # PAB4865 ) . 293 cell lysate ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the AGR2 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of AGR2 polyclonal antibody ( Cat # PAB4865 ) in NCI-H460 cell lysate ( 35 µg/lane ) . AGR2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
AGR2 polyclonal antibody ( Cat # PAB7143 ) ( 0.01 µg/ml ) staining of human duodenum lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
TA303161 (0.01µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of AGR2 in Hela cell lysate with AGR2 antibody at (A) 1 and (B) 2 ug/mL
Immunohistochemistry of AGR2 in human small intestine tissue with AGR2 antibody at 5 ug/mL.
Immunofluorescence of AGR2 in human small intestine tissue with AGR2 antibody at 20 ug/mL.
Predicted band size: 20kDa. Positive control: Mouse large intestine tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1000 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 20 seconds)
Predicted cell location: Cytoplasm. Positive control: Human renal cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using AGR2 antibody at dilution 1/10, on the right is treated with the fusion protein.(Original magnification:x100)
Predicted band size: 20kDa. Positive control: Mouse large intestine tissue lysate. Recommended dilution: 1/1000-5000
Predicted cell location: Cytoplasm. Positive control: Human renal cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using AGR2 antibody at dilution 1/10, on the right is treated with the fusion protein.(Original magnification:x200)
AGR2 Antibody (N-term) (Cat. #TA324827) western blot analysis in HT29,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat.#TA324827), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of A549 cell stained with AGR2 Antibody (N-term)(Cat#TA324827).A549 cells were incubated with AGR2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . AGR2 immunoreactivity is localized to Cytoplasm significantly.
AGR2 Antibody (Center) (Cat. #TA324828) western blot analysis in HT29,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).
AGR2 Antibody (Center) (Cat. #TA324828) western blot analysis in HT29,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).
Western blot analysis of lysates from ZR-75-1, HT29 cell line (from left to right), using AGR2 Antibody (Center)(Cat. #TA324828). TA324828 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemistry-Paraffin: AGR2 Antibody [TA336509] - Immunohistochemical staining with TA336509, anti-AGR2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.
Immunohistochemistry: AGR2 Antibody [TA336715] - Detection of AGR2 in mouse kidney.
Immunohistochemistry-Paraffin: AGR2 Antibody [TA336715] - Detection of AGR2 in mouse prostate.
Western Blot: AGR2 Antibody [TA336716] - Human stomach lysate.
Immunohistochemistry: AGR2 Antibody [TA336716] - Detection of AGR2 in mouse kidney.
Immunohistochemistry: AGR2 Antibody [TA336716] - Detection of AGR2 in mouse prostate.
Immunocytochemistry/Immunofluorescence: AGR2 Antibody [TA336716] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: AGR2 Antibody (10E2) [TA337147] - analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse I
Immunohistochemistry-Paraffin: AGR2 Antibody (10E2) [TA337147] - Formalin-fixed, paraffin-embedded human breast stained with AGR2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer
Immunohistochemistry-Paraffin: AGR2 Antibody (10E2) [TA337147] - Formalin-fixed, paraffin-embedded human spleen tissue stained with 5 ug/ml concentration of AGR2 antibody .
Immunohistochemistry-Paraffin: AGR2 Antibody (10E2) [TA337147] - IHC-P detection of AGR2 protein in a human ovarian cancer section using 5 ug/ml concentration of AGR2 antibody. The representative image shows AGR2 immuno-reactivity in the developing ovaria
Immunohistochemistry-Paraffin: AGR2 Antibody (10E2) [TA337147] - IHC-P detection of AGR2 protein in a section of human bladded cancer using 5 ug/ml concentration of AGR2 antibody. The AGR2 immunoreactivity was observed in cellular cytoplasm and the inter-
Immunohistochemistry-Paraffin: AGR2 Antibody (10E2) [TA337147] - paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffe
Western Blot: AGR2 Antibody [TA337169] - analysis of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate © probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at
Immunohistochemistry-Paraffin: AGR2 Antibody [TA337169] - analysis of AGR2 using AGR2 antibody. Formalin-fixed, paraffin-embedded normal colon tissue probed with AGR2 antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: AGR2 Antibody [TA337169] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the breast stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced
Immunohistochemistry-Paraffin: AGR2 Antibody [TA337169] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337147 | AGR2 antibody |
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| Mouse Monoclonal AG-2 Antibody (10E2) | |||||||
![Western Blot: AGR2 Antibody (10E2) [TA337147] - analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse I](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-27175-1-W.jpg) |
Mouse | 1E3 | Purified | Bov, Can, Eq, Hu, Ms | C, P, WB |
0.1 mg /
$415.00
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| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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