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AATF antibody

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Principal name

AATF antibody

Alternative names for AATF antibody

CHE1, DED, HSPC277, CHE-1, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1

SwissProt ID

Q9JKX4 (Mouse), Q9NY61 (Human), Q9QYW0 (Rat)

Gene ID

26574 (AATF), 56321 (Aatf), 114512

Ncbi ID

NP_036270, 9606, NP_036270.1, NM_019816, NM_053720, NP_062790.1

Available reactivities

Can (Canine), Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of AATF antibody

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK) (1), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases (2). AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways (3). Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the b-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD) (4,5), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

General readings

Burgdorf,S., et al., (2004) J. Biol. Chem. 279 (17), 17524-17534

ARP31867_P050 - AATF
Human Intestine; AATF antibody - N-terminal region (ARP31867_P050) in Human Intestine cells using Immunohistochemistry
ARP31867_P050 - AATF
Human Liver; AATF antibody - N-terminal region (ARP31867_P050) in Human Liver cells using Immunohistochemistry
ARP31867_P050 - AATF
Human kidney; WB Suggested Anti-AATF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human kidney; AATF antibody - N-terminal region (ARP31867_P050) in Human kidney cells using Western Blot
H00026574-M09 - AATF
Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml]
H00026574-M09 - AATF
AATF monoclonal antibody (M09), clone 2H6 Western Blot analysis of AATF expression in HeLa ( Cat # L013V1 ).
H00026574-A01 - AATF
AATF polyclonal antibody (A01), Lot # AUH0060323QCS1 Western Blot analysis of AATF expression in PC-12 ( Cat # L012V1 ).
ARP38957_T100 - AATF
Human Daudi; WB Suggested Anti-AATF Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. AATF is supported by BioGPS gene expression data to be expressed in Daudi.; AATF antibody - N-terminal region (ARP38957_T100) in Human Daudi cells using Western Blot
ARP38958_T100 - AATF
Human kidney; WB Suggested Anti-AATF Antibody Titration: 0.25ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney; AATF antibody - C-terminal region (ARP38958_T100) in Human kidney cells using Western Blot
AP07651PU-N - AATF
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis.
H00026574-M04 - AATF
Immunoperoxidase of monoclonal antibody to AATF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
H00026574-M04 - AATF
Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml]
H00026574-M04 - AATF
AATF monoclonal antibody (M04), clone 3C7 Western Blot analysis of AATF expression in Hela NE ( Cat # L013V3 ).
PAB12886 - AATF
Immunohistochemistry of AATF in human small intestine tissue with AATF polyclonal antibody ( Cat # PAB12886 ) at 10 µg/ml.
PAB12886 - AATF
Western blot analysis of AATF in human small intestine tissue lysate with AATF polyclonal antibody ( Cat # PAB12886 ) at 0.5, 1 and 2 µg/ml.
C11778-1 - AATF
Western blot analysis of extracts from HeLa cells/HepG2 cells/COLO205 cells/HuvEc cells, using AATF Antibody. The lane on the right is treated with the synthesized peptide.
CPA2614-100ul - AATF
Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2614-100ul - AATF
Immunofluorescent analysis of AATF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2614-100ul - AATF
Western blot analysis of AATF expression in HUVEC (A); HeLa (B) whole cell lysates.
TP710286 - AATF
TA306107 - AATF
Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
TA306107 - AATF
Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 ug/ml.
TA306107 - AATF
Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 ug/mL.
TA312747 - AATF
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO205 cells and HUVEC cells, using AATF antibody.The lane on the right is treated with the synthesized peptide.
TA343683 - AATF
WB Suggested Anti-AATF Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 312500; Positive Control: Daudi cell lysate. AATF is supported by BioGPS gene expression data to be expressed in Daudi
TA343684 - AATF
WB Suggested Anti-AATF Antibody Titration: 0.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human kidney
TA350967 - AATF
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, 231 cells, Primary antibody: (AATF Antibody) at dilution 1/667, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
TA329431 - AATF
WB Suggested Anti-AATF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human kidney
TA329431 - AATF
Human Intestine
TA329431 - AATF
Human Liver

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AATF antibody
  Rabbit Polyclonal AATF Antibody  
Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / $415.00
  OriGene Technologies, Inc.
+2 additional images

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  3. Western blot / Immunoblot (WB) (1)
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