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AADAT antibody

Principal name

AADAT antibody

Alternative names for AADAT antibody

KAT2, Kynurenine aminotransferase II, Kynurenine-oxoglutarate aminotransferase II, Kynurenine-oxoglutarate transaminase II, 2-aminoadipate transaminase, 2-aminoadipate aminotransferase, Alpha-aminoadipate aminotransferase

SwissProt ID

Q64602 (Rat), Q8N5Z0 (Human), Q9WVM8 (Mouse)

Gene ID

51166 (AADAT), 23923 (Aadat), 29416

Ncbi ID

9606, NP_057312, NP_057312.1, NP_872603, NP_035964.1, NM_011834, NM_017193, NM_182662, NM_016228

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), GP (Guinea Pig), Ye (Yeast)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of AADAT antibody

AADAT is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties.This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

General readings

Goh DL, Patel A, Thomas GH, Salomons GS, Schor DS, Jakobs C, Geraghty MT.
Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT).
Mol Genet Metab. 2002 Jul;76(3):172-80.
PMID: 12126930

Human kidney; AADAT antibody - N-terminal region (ARP43534_T100) in Human kidney cells using Immunohistochemistry

ARP43534_T100

Human HepG2; WB Suggested Anti-AADAT Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; AADAT antibody - N-terminal region (ARP43534_T100) in Human HepG2 cells using Western Blot

ARP43534_T100

AADAT MaxPab polyclonal antibody. Western Blot analysis of AADAT expression in human liver.

H00051166-B01P

Western Blot analysis of AADAT expression in transfected 293T cell line (H00051166-T01) by AADAT MaxPab polyclonal antibody.Lane 1: AADAT transfected lysate(47.19 KDa).Lane 2: Non-transfected lysate.

H00051166-B01P

Human COLO205; WB Suggested Anti-AADAT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate; AADAT antibody - middle region (ARP43535_P050) in Human COLO205 cells using Western Blot

ARP43535_P050

AADAT Antibody (Center) (Cat. #AP50010PU-N) western blot analysis in HepG2,Y79 cell line lysates (35ug/lane).This demonstrates the AADAT antibody detected the AADAT protein (arrow)

AP50010PU-N

TP322252

TP323277

TP323277

TA303317 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303317

Western blot analysis of THP1 cells (Lanes 1 and 2: 60ug lysate) and Jurkat cells (Lane 3: 80ug lysate) with anti-AADAT antibody diluated at 1:2000. (Predicted and observed size: 47kDa)

TA310138

Anti-AADAT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA317921

Anti-AADAT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA317922

Anti-KATII / AADAT antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA317922

Anti-KATII / AADAT antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.