Product Description for NT5C3
Rabbit anti Bovine, Canine, Chicken, Human, Mouse, Rabbit, Rat, Zebrafish, African clawed frog NT5C3.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties for NT5C3
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | Cytosolic 5'-nucleotidase 3, Cytosolic 5'-nucleotidase III, P5N1, Pyrimidine 5'-nucleotidase 1, UMPH1, Uridine 5'-monophosphate hydrolase 1, p36 |
| Presentation | Aff - Purified |
| Reactivity | African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt, Ze |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western blotting (0.2 - 1 µg/ml) |
| Background | Pyrimidine 5-prime-nucleotidase (P5N), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology. Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology. |
| General Readings | Amici,A., et al., (2002) Genomics Meth. Enzymol. 354,149-159 |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for NT5C3 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP305629 |
NT5C3 (transcript variant 3) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP309741 |
NT5C3 (transcript variant 2) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
Positive controls for NT5C3 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY424319 |
NT5C3A overexpression lysate |
||||||
 |
0.1 mg /
$295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| LY425069 |
NT5C3A overexpression lysate |
||||||
|
0.1 mg /
$295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for NT5C3 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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