Product Description for Inositol monophosphatase 3 / IMPA3
Inositol monophosphatase 3 / IMPA3.
Presentation: Purified
Presentation: Purified
Properties for Inositol monophosphatase 3 / IMPA3
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 50 µg |
| Synonyms | IMPAD1, IMPase 3, Inositol monophosphatase domain-containing protein 1, Inositol-1(or 4)-monophosphatase 3, Myo-inositol monophosphatase A3 |
| Presentation | Purified |
| Molecular weight | 37.6 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 90 % by SDS - PAGE |
| Background | IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Protocols | 1. Prepare 1mM PAP in 20mM Tris-HCl (PH 7.5), 15mM MgCl2.2. Dilute IMPAD1 various concentration (20ug/ml, 10ug/ml, 5ug/ml) in 20mM Tris-HCl (PH 7.5), 15mM MgCl2, containing blank of 20mM Tris-HCl, 15mM MgCl2 3. Make the Phosphate standard range of 0.31 nmol to 20 nmol per well.4. Load 25 ul of 1mM PAP add 25 ul of diluted IMPAD1 to well and load 50 ul of phosphate standard.5. Incubate for15 minute at 25C.6. Add 150 ul of Malachite green solution mix and incubate for 15 minute at 25C.7. Read at 650nm.- Malachite green solution: Ammonium molybdate 675 mg, Malachite green carbinol hydrochloride 3.3 mg in 50 ml of 1M H2SO4. |
| General Readings | Vissers L E., et al. (2011) Am J Hum Genet. 88:608-615. Kalujnaia S., et al. (2010) FASEB J. 24:3981-3991. |
| Storage | Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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