discontinued

BM145 Myelin antibody

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Quick Overview

Mouse anti Human Myelin PM43

BM145

Product Description for Myelin

Mouse anti Human Myelin PM43.
Presentation: Ascites
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Myelin

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Ascites
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone PM43
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
43 kDa protein isolated from the PNS. Spleen cells from immunised Balb/c mice were fused with cells of the Sp 2/0 myeloma cell line.
Application Western Blot.
Immunohistochemistry on frozen and paraffin sections.
Recommended Positive Control: Peripheral nerve.
Background Myelin is produced by oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS). Defining the differences between CNS and PNS myelin may provide insights into the process of myelination and the pathogenesis of certain idiopathic diseases involving myelin such as multiple sclerosis, certain leukodystrophies, and other myelinopathies.
Myelin is composed of about 80% lipid fat and about 20% protein. Some of the proteins that make up myelin are Myelin basic protein (MBP), Myelin oligodendrocyte glycoprotein (MOG) and Proteolipid protein (PLP). Myelin is made up primarily of a glycolipid called galactocerebroside. The intertwining of the hydrocarbon chains of sphingomyelin serve to strengthen the myelin sheath.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Ascites containing 0.09% Sodium Azide
Ascites
Specificity
Specificity:
This antibody is specific for myelin. In paraffin sections it recognises only myelin in the peripheral nervous system. In frozen sections it will aslo weakly stain myelin in the central nervous system. In Western blotting this antibody is directed agaisnt a 43 kDa protein.

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