NANOG (1-154, His-tag) - AR39064PU-N

Product Description for NANOG

NANOG.
Presentation: Purified

Immunogen	Swiss Prot Num: Q9H9S0
Purity	> 90 %
Background	NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed.
Concentration	0.25 mg/ml (determined by Bradford assay)
General Readings	Clark A T., et al. (2004) Stem Cells. 22:169-179. Chambers I., et al. (2003) Cell Res. 13:499-502.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ` Molecular weight: (174aa), confirmed by MALDI-TOF<br />(Molecular weight on SDS-PAGE will appear higher).
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT Purity: >90% State: Liquid purified protein Purified