Product Description for Peroxiredoxin-3 / PRDX3
Peroxiredoxin-3 / PRDX3.
Presentation: Purified
Presentation: Purified
Properties for Peroxiredoxin-3 / PRDX3
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | AOP-1, AOP1, Antioxidant protein 1, HBC189, PRDX-3, PRX-III, Protein MER5 homolog, Prx-3, Prx3, Thioredoxin-dependent peroxide reductase mitochondrial |
| Presentation | Purified |
| Molecular weight | 21.5 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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| Purity | > 95 % by SDS - PAGE |
| Background | Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay: 1.Prepare a 50ul reaction mix into a suitable container : The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2.Add 5 ul of recombinant PRDX3 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3.Incubate at 25°C for 2 minutes. 4.Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5.Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6.Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) 7.Record the increase in A475nm. |
| General Readings | Cox AG., et al. (2009). Biochemistry. 48(27):6495-501. Rhee SG., et al. (2005). Free Radic Biol Med. 38(12):1543-52. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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