Product Description for NFYA
Rabbit anti Human, Mouse NFYA.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Electrophoretic Mobility Shift Assay, Immunoprecipitation, Western blot / Immunoblot.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Electrophoretic Mobility Shift Assay, Immunoprecipitation, Western blot / Immunoblot.
Properties for NFYA
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.1 mg |
| Synonyms | Nuclear transcription factor Y subunit alpha |
| Presentation | Purified |
| Reactivity | Hu, Ms |
| Applications | E, EMSA, IP, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilution(s): This product was assayed by Immunoblot and found to be reactive against the differentially spliced 35 and 42 kDa forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP). No reaction was observed by immunoblot against the 25 kDa B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 µl per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP) and ABTS substrate. A dilution of 1:10,000 is suggested from this experiment. |
| Background | NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information. |
| Concentration | 1.1 mg/ml (by UV absorbance at 280 nm) |
| Storage | Store the antibody (in aliquots) at -20°C. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
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Accessory Products
Proteins and/or Positive Controls
Proteins for NFYA (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP760255 |
NFYA (full length, N-term HIS tag, transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
$205.00
|
|||
| OriGene Technologies, Inc. | |||||||
Positive controls for NFYA (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY400893 |
NFYA overexpression lysate |
||||||
 |
0.1 mg /
$295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for NFYA (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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