R1402AP Human Lambda Light Chain (F(ab)2 Fragment) antibody

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0.5 mg / $340.00
Delivery Time: 2-3 working days*
(*) valid for US customers only

Quick Overview

Goat anti Human Lambda Light Chain

R1402AP

Product Description for Human Lambda Light Chain

Goat anti Human Lambda Light Chain.
Properties: (F(ab)2 Fragment)
Presentation: AP

Properties for Human Lambda Light Chain

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Presentation AP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human lambda chain
Property F(ab)2 Fragment
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
Storage Store the antibody as an undiluted liquid at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
State:
Liquid (sterile filtered) F(ab')2 fragments.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Alkaline Phosphatase and anti-Goat Serum.
No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.

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