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SM014RT CD11b antibody

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25 Tests / $220.00
Delivery Time: 3-5 working days*
(*) valid for US customers only

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Quick Overview

Rat anti Human, Mouse, Rabbit CD11b M1/70.15

SM014RT

Product Description for CD11b

Rat anti Human, Mouse, Rabbit CD11b M1/70.15.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for CD11b

Product Category Primary Antibodies
Target Category
Quantity 25 Tests
Synonyms CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Presentation PE
Reactivity Hu, Ms, Rb
Applications F
Clonality Monoclonal
Clone M1/70.15
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05555
Immunogen:
T cell enriched splenocytes from B10 mice.
Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Isotype control SM19R
Application Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Background CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages.
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
General Readings 1. Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three
complement receptor. J Exp Med. 156 (4): 1000-9.
2. FernándezSuárez, D. (2014) The monoacylglycerol lipase inhibitor JZL184 is neuroprotective
and alters glial cell phenotype in the chronic MPTP mouse model Neurobiol Aging. 35: 2603-16.
3. Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 20 (12): 2553-8.
4. Terrando, N. et al. (2010) The impact of IL-1 modulation on the development of lipopolysaccharide-induced cognitive dysfunction. Crit Care. 14 (3): R88.
5. Redensek, A. et al. (2011) Expression and detrimental role of hematopoietic prostaglandin D synthase in spinal cord contusion injury. Glia. 59: 603-14.
6. Brochard, V. et al (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease. J Clin Invest. 119: 182-92.
7. Chinnery, H.R. et al. (2010) Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice.
J Neuropathol Exp Neurol. 69: 896-909.
8. Ferger, A.I. et al (2010) Effects of mitochondrial dysfunction on the immunological properties of microglia. J Neuroinflammation. 7: 45.
9. Gales, A. et al (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans. PLoS Pathog. 6 : e1000714.
10. Geier, H. and Celli, J. (2011) Phagocytic Receptors Dictate Phagosomal Escape and Intracellular Proliferation of Francisella tularensis. Infect Immun. 79: 2204-14.
11. Ghasemlou, N. et al. (2010) Mitogen-activated protein kinase-activated protein kinase 2
(MK2) contributes to secondary damage after spinal cord injury. J Neurosci. 30: 13750-9.
12. Huang, Q.Q. et al (2008) Role of H2-calponin in regulating macrophage motility and phagocytosis.
J Biol Chem. 283: 25887-99.
13. Hudcovic, T. et al (2009) Monocolonization with Bacteroides ovatus protects
immunodeficient SCID mice from mortality in chronic intestinal inflammation caused by
long-lasting dextran sodium sulfate treatment. Physiol Res. 58: 101-10.
14. Kanu, N. et al. (2010) The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain. J Biol Chem. 285: 38534-42.
15. Kapturczak, M.H. et al (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse. Am J Pathol. 165: 1045-53.
16. Kroner, A. et al (2010) Ectopic T-cell specificity and absence of perforin and granzyme B alleviate neural damage in oligodendrocyte mutant mice. Am J Pathol. 176: 549-55.
17. L'Episcopo, F. et al. (2010) Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl- 4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease. J Neuroinflammation. 7: 83.
18. Samanta, J. et al. (2010) Noggin protects against ischemic brain injury in rodents.
Stroke.41: 357-62.
19. Yang, X. et al (2010) The role of the JAK2-STAT3 pathway in pro-inflammatory responses of
EMF-stimulated N9 microglial cells. J Neuroinflammation. 7: 54.
20. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy. J Neurosci. 31: 3610-24.
21. Macrez, R. et al. (2016) Neuroendothelial NMDA receptors as therapeutic targets in
experimental autoimmune encephalomyelitis. Brain. Jul 19. pii: aww172. [Epub ahead of print] 
22. Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration. Assay Drug Dev Technol. Jul 8. [Epub ahead of print]
23. Werneburg, S. et al. (2016) Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages. Glia. 64 (8): 1314-30.
24. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke. Pharmacol Res. 102: 298-307.
25. Chen, Z.Z. et al. (2016) Memantine mediates neuroprotection via regulating neurovascular
unit in a mouse model of focal cerebral ischemia. Life Sci. 150: 8-14.
26. Rich, M.C. et al. (2016) Site-targeted complement inhibition by a complement receptor
2-conjugated inhibitor (mTT30) ameliorates post-injury neuropathology in mouse brains.
Neurosci Lett. 617: 188-94.
27. McCarthy, R.C. et al. (2016) Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J Neuroinflammation. 13: 21.
28. Jones, R.S. et al. (2015) Inhibition of JAK2 attenuates the increase in inflammatory markers in microglia from APP/PS1 mice. Neurobiol Aging. 36 (10): 2716-24.
29. Amantea, D. et al. (2016) Azithromycin protects mice against ischemic stroke injury by promoting macrophage transition towards M2 phenotype. Exp Neurol. 275 Pt 1: 116-25.
30. Bains, M. & Roberts, J.L. (2016) Estrogen protects against dopamine neuron toxicity in primary mesencephalic cultures through an indirect P13K/Akt mediated astrocyte pathway.
Neurosci Lett. 610: 79-85.
31. Ji, J. et al. (2015) Iptakalim protects against ischemic injury by improving neurovascular unit function in the mouse brain. Clin Exp Pharmacol Physiol. 42 (7): 766-71.
32. Kim, B.W. et al. (2015) αAsarone attenuates microgliamediated neuroinflammation by inhibiting NF kappa B activation and mitigates MPTP-induced behavioral deficits in a mouse model of Parkinson's disease. Neuropharmacology. 97: 46-57.
Storage Prior to and following reconstitution store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide, 1% BSA, 5% Sucrose
State:
Lyophilized purified IgG fraction.
Reconstitution:
Restore with distilled water.
Specificity
Specificity:
This antibody recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1).
Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose.
Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
Species:
Human, Mouse and Rabbit.

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