Product Description for Bcl-2
Rabbit anti Human Bcl-2.
Properties: pThr56
Presentation: Azide Free
Product is tested for Enzyme Immunoassay.
Properties: pThr56
Presentation: Azide Free
Product is tested for Enzyme Immunoassay.
Properties for Bcl-2
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.5 mg |
| Synonyms | Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha |
| Presentation | Azide Free |
| Reactivity | Hu |
| Applications | E |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | pThr56 |
| Application | ELISA: Reacts with synthetic peptide (SQPGHTpPHPASR) coated plate. |
| Background | Bcl-2 is a 25kD inner mitochondrial membrane protein with anti-apoptotic function. It is believed that Bcl-2 suppresses apoptosis by inhibiting mitochondrial cytochrome C release, blocking caspase cascade and regulating mitochondrial calcium homeostasis. Bcl- 2 is identified from the study of B-cell lymphoma. About 90% of follicular lymphoma is caused by Bcl-2 overexpression resulted from the translocation of Bcl-2 gene from chromosome 18 to the immunoglobulin heavy chain enhancer on chromosome 14. Bcl-2 is also involved with other malignancies such as melanoma, breast cancer, prostate cancer and lung cancer. Bcl-2 Thr56 phosphorylation by p38 MAPK results in cytochrome C release. Mutation near Thr56 aborted Bcl-2 antiapoptotic function. |
| Storage | Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
Accessory Products
Proteins and/or Positive Controls
Proteins for Bcl-2 (6 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP304498 |
Bcl-2 (transcript variant alpha) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR09177PU-L |
Bcl-2 (1-211, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
$1,070.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09177PU-N |
Bcl-2 (1-211, His-tag) |
||||||
|
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
$400.00
|
||
| OriGene Technologies GmbH | |||||||
| AR10283PU-L |
Bcl-2 (des BH4 Domain, His-tagged) |
||||||
|
Human | Purified |
> 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10283PU-N |
Bcl-2 (des BH4 Domain, His-tagged) |
||||||
|
|
Human | Purified |
> 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10283PU-S |
Bcl-2 (des BH4 Domain, His-tagged) |
||||||
|
|
Human | Purified |
> 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
Recommended Secondary Antibodies for Bcl-2 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
Compare Products
You have no items to compare.
My Cart
You have no items in your shopping cart.
