Product Description for APOBEC3A
Goat anti Human APOBEC3A.
Presentation: Aff - Purified
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections, Enzyme Immunoassay.
Presentation: Aff - Purified
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections, Enzyme Immunoassay.
Properties for APOBEC3A
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.1 mg |
| Synonyms | Apolipoprotein B mRNA editing enzyme, Phorbolin-1, catalytic polypeptide-like 3A |
| Presentation | Aff - Purified |
| Reactivity | Hu |
| Applications | E, F, ICC/IF, P, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Peptide ELISA: 1/8000 (Detection limit). Western blot: 3-6 µg/ml. Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature. Immunohistochemitry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus of A431 and A549 cells. Flow Cytometry: 10 µg/ml. Flow cytometric analysis of Jurkat cells. |
| Background | PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene. |
| Concentration | 0.5 mg/ml |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. |
| Format |
|
| Specificity |
|
| Gene ID | 200315 |
Accessory Products
Proteins and/or Positive Controls
Proteins for APOBEC3A (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP320995 |
APOBEC3A |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
Positive controls for APOBEC3A (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY407901 |
APOBEC3A overexpression lysate |
||||||
 |
0.1 mg /
$295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for APOBEC3A (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1317AP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317B |
Goat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317F |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1317HRP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317T |
Goat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | TRITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1435C2 |
Goat IgG (H+L chain), adsorbed antibody |
||||||
|
|
Donkey | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1435C3 |
Goat IgG (H+L chain), adsorbed antibody |
||||||
|
|
Donkey | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1435C5 |
Goat IgG (H+L chain), adsorbed antibody |
||||||
|
|
Donkey | Cy5 | F, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
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