TERT antibody
Principal name
TERT antibody
Alternative names for TERT antibody
Telomerase reverse transcriptase, HEST2, EST2, TCS1, TRT, Telomerase catalytic subunit, Telomerase-associated protein 2
Ncbi ID
9606, NP_001180305.1, NP_001180305, NP_033380.1, NM_198253, NM_009354, NM_053423, NM_001193376
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of TERT antibody
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays ictive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within R component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt sigling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, corory artery disease (CAD), dyskeratosis congenita autosomal domint type 2 (DKCA2), pulmory fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmory fibrosis idiopathic (IPF).
Western blot analysis of TERT polyclonal antibody ( Cat # PAB2490 ) in Jurkat cell line lysates ( 35 µg/lane ) . TERT ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Dot blot analysis of TERT (phospho S1125) polyclonal antibody ( Cat # PAB4934 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Dot blot analysis of anti-Phospho-TERT pSer1125 Antibody (Cat.#AP12956PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Dot blot analysis of anti-Phospho-TERT-pY707 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of human TERT using TERT monoclonal antibody, clone 2D8 ( Cat # MAB2379 ). Sample : MJ90 whole cell lysate.
Western blot analysis of TERT in HeLa nuclear extracts with TERT polyclonal antibody ( Cat# PAB12002 ).
Staining of Skeletal Muscle lysate (35µg protein in RIPA buffer) using TERT Antibody Cat.-No AP31849PU-N at 0.5 µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of Telomerase expression in Jurkat (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Fluorescent confocal image of SY5Y cells stained with TERT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated TERT primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
Fluorescent confocal image of HeLa cell stained with TERT Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
TERT Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot of anti-TERT Antibody in Jurkat cell line lysates (35 ug/lane). TERT(arrow) was detected using the purified antibody.
Formalin-Fixed, Paraffin-Embedded Human Skin tissue stained with Anti-TERT / Telomerase antibody Cat.-No AP33476PU-N at 1/100 dilution after heat-induced antigen retrieval.
Western Blot analysis of human TERT
Western Blot analysis of human TERT 1:500 dilution on MJ90 cells
Normal pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans
Immunocytochemistry/Immunofluorescence: Telomerase reverse transcriptase Antibody (2C4) - Detection of Tert (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33258.
Flow Cytometry: Telomerase reverse transcriptase Antibody (2C4) - Staining of NTERA-2 cells at a 1:200 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.
Immunofluorescence analysis of HUVEC cells, using Telomerase antibody.The picture on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TERT antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x200)
Predicted cell location: Cytoplasm . Positive control: Human tonsil and esophagus cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using TERT antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm . Positive control: Human tonsil and esophagus cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using TERT antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm or Nucleus . Positive control: Human thyroid and ovarian cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using TERT antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm or Nucleus . Positive control: Human thyroid and ovarian cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using TERT antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of anti-TERT Antibody (Center) (RB14069) in Jurkat cell line lysates (35ug/lane). TERT(arrow) was detected using the purified Pab.
IF image of Hela cell stained with TERT Antibody (Center)(Cat#TA324535).Hela cells were incubated with TERT primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
IF image of SY5Y cells stained with TERT (Center) antibody. SY5Y cells were incubated with TA324535 TERT (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . TERT immunoreactivity is localized to the nucleus of SY5Y cells.
TERT Antibody (Center) (Cat. #TA324535) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: Telomerase reverse transcriptase Antibody [TA336564] - Detection of Telomerase reverse transcriptase in HeLa nuclear extracts using TA336564.
Immunocytochemistry/Immunofluorescence: Telomerase reverse transcriptase Antibody [TA336564] - The Telomerase reverse transcriptase antibody was tested in V6.5 mouse embryonic stem cells (cat# NBP1-41162) against Dylight 488. Actin and nuclei were counte
Western blot analysis of TERT expression in Jurkat cell extracts
Western blot analysis of Telomerase (Phospho-Ser824) expression in Jurkat cell extracts
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31849PU-N | TERT (597-611) antibody |
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
$380.00
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| AP12956PU-N | TERT pSer1125 antibody |
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Rabbit | Ig | Aff - Purified | Hu |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
| AP13029PU-N | TERT pTyr707 antibody |
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Rabbit | Ig | Aff - Purified | Hu |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||