SSBP1 antibody
Principal name
SSBP1 antibody
Alternative names for SSBP1 antibody
SSBP, Mt-SSB, mtSSB, Single-stranded DNA-binding protein mitochondrial
SwissProt ID
P28042 (Rat), P54622 (Drome), Q04837 (Human), Q32PB0 (Bovin), Q84J78 (Arath), Q95KK4 (Rabit), Q9CYR0 (Mouse)
Ncbi ID
9606, NP_003134, NP_003134.1, NM_003143, NP_082634.1, NM_212468, NM_028358, NM_183328
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SSBP1 antibody
- Kit Component:
- - KN316756G1, Ssbp1 gRNA vector 1 in pCas-Guide vector
- KN316756G2, Ssbp1 gRNA vector 2 in pCas-Guide vector
- KN316756D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Müller-Höcker J, et al. (1993). Appl Immunohistochem Mol Morphol. 9(3):276-80.Tiranti V, Rocchi M, et al. (1993). Gene. 126(2):219-25.
Detection limit for recombinant GST tagged SSBP1 is approximately 0.3ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes of SSBP1 antibody (Cat.-No.: AP01371PU-N) in paraffin-embedded human pancreas tissue
Western blot (WB) analysis of SSBP1 antibody (Cat.-No.: AP01371PU-N) in extracts from HUVEC.
Human Fetal Kidney; WB Suggested Anti-SSBP1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Kidney; SSBP1 antibody - N-terminal region (ARP61972_P050) in Human Fetal Kidney cells using Western Blot
Hum. Fetal Heart; Host:Rabbit. Target Name:SSBP1. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; SSBP1 antibody - N-terminal region (ARP61972_P050) in Hum. Fetal Heart cells using Western Blot
Immunohistochemical analysis of SSBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SSBP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of SSBP1 expression in A375 (A); HeLa (B); MOLT4 (C) whole cell lysates.
Western blot analysis of extracts from HeLa cells and HUVEC cells, using MtSSB antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using MtSSB antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using MtSSB antibody.The picture on the right is treated with the synthesized peptide.
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-5: PC3 cells, A549 cells, K562 cells, A375 cells, hela cells, Primary antibody: (SSBP1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (SSBP1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (SSBP1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP01371PU-N | SSBP1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
$370.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||