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RAB3IL1 antibody

Principal name

RAB3IL1 antibody

Alternative names for RAB3IL1 antibody

Rabin3-like 1, GRAB, Guanine nucleotide exchange factor for Rab-3A, Rab-3A-interacting-like protein 1

SwissProt ID

Q2KJ58 (Bovin), Q8TBN0 (Human), Q8VDV3 (Mouse), Q99NH3 (Rat)

Gene ID

5866 (RAB3IL1), 74760 (Rab3il1), 171452

Ncbi ID

9606, NP_037533, NP_037533.2, NM_013401, NP_653121.1, NM_144538, NM_134411

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of RAB3IL1 antibody

Kit Component:: - KN314369G1, Rab3il1 gRNA vector 1 in pCas-Guide vector
- KN314369G2, Rab3il1 gRNA vector 2 in pCas-Guide vector
- KN314369D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Huang H C., et al. (2000) Proc Natl Acad Sci uSA. 97: 12475-12480.Tsai P Y., et al. (2012) Arthritis Rheum. 64: 232-242.

Human Muscle; WB Suggested Anti-RAB3IL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; RAB3IL1 antibody - middle region (ARP56739_P050) in Human Muscle cells using Western Blot

ARP56739_P050

Human Lung; WB Suggested Anti-RAB3IL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; RAB3IL1 antibody - middle region (ARP56740_P050) in Human Lung cells using Western Blot

ARP56740_P050

Western blot analysis of RAB3IL1 Antibody (C-term) Cat.-No AP53546PU-N in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RAB3IL1 antibody detected the RAB3IL1 protein (arrow).

AP53546PU-N

Flow cytometric analysis of NCI-H460 cells using RAB3IL1 Antibody (C-term) Cat.-No AP53546PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP53546PU-N

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti- RAB3IL1monoclonal antibody.(1:200)

TA504958

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3IL1 (RC204592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB3IL1.

TA504958

HEK293T cells transfected with either RC204592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RAB3IL1 antibody(TA504958), and then analyzed by flow cytometry.