POU2AF1 / BOB1 antibody
Principal name
POU2AF1 / BOB1 antibody
Alternative names for POU2AF1 / BOB1 antibody
OBF1, OBF-1, OCA-B, OCAB, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, OCT-binding factor 1
Ncbi ID
9606, NP_006226, NP_006226.2, NP_035266.1, NM_006235, NM_011136
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of POU2AF1 / BOB1 antibody
POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.
General readings
Strubin M, Newell JW, Matthias P.
OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.
Cell. 1995 Feb 10;80(3):497-506.
PMID: 7859290
POU2AF1 polyclonal antibody ( Cat # PAB6265 ) staining ( 2 µg/ml ) of mouse spleen lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human tonsil stained with Anti-Bob-1 antibody (Cat# AP15373PU).
Immunohistochemical staining of POU2AF1 on formalin fixed, paraffin embedded human tonsil with POU2AF1 polyclonal antibody.
AM12071PU Bob-1 antibody staining of Human Tonsil.
AM12071PU Bob-1 antibody staining of Ramos cell lysate by Western Blot.
Recombinant human POU2AF1, 1-256aa, His-tagged
Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) cat.no. AP53397PU-N for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of POU2AF1 antibody (N-term) cat.no. AP53397PU-N in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab.
Flow cytometry analysis of Ramos cells (bottom histogram) using POU2AF1 antibody cat.no. AP53397PU-N compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Paraffin Embedded Human Spleen using POU2AF1 / BOB1 Antibody Cat.-No AP32080PU-N shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells.
HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with POU2AF1 Antibody Cat.-No AP32080PU-N (1 µg/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/3000) in Lane C. Mock-transfected HEK293 probed with POU2AF1 Antibody AP32080PU-N (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with POU2AF1 Antibody Cat.-No AP32714PU-N (0.5 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with AP32714PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Staining of Daudi lysate using POU2AF1 Antibody Cat.-No AP32714PU-N at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.
ELISA: BOB1 Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-POU2AF1 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807917)(1:150) Dilut
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807917)(1:150) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807917)(1:150) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807917)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807923)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807923)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807926)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807930)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807930)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody at 1:150.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807990)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POU2AF1 mouse monoclonal antibody at 1:150. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807990
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807999)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807999)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
TA302834 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Predicted cell location: Nucleus. Positive control: Human breast and thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using POU2AF1 antibody at dilution 1/15, on the right is treated with the synthetic peptide.(Originalmagnification:x200)
Predicted cell location: Nucleus. Positive control: Human breast and thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using POU2AF1 antibody at dilution 1/15, on the right is treated with the synthetic peptide.(Originalmagnification:x200)
POU2AF1 Antibody (Cat. #TA328146) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the POU2AF1 antibody detected the POU2AF1 protein (arrow).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590578 | POU2AF1 / BOB1 antibody |
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| Rabbit Polyclonal BOB1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
$409.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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