PHGDH antibody
Principal name
PHGDH antibody
Alternative names for PHGDH antibody
PGDH3, 3-PGDH, D-3-phosphoglycerate dehydrogenase
SwissProt ID
A5GFY8 (Pig), O04130 (Arath), O08651 (Rat), O43175 (Human), P0A9T0 (Ecoli), P40054 (Yeast), P87228 (Schpo), Q5EAD2 (Bovin), Q61753 (Mouse)
Ncbi ID
9606, NP_006614, NP_006614.2, NP_058662.2, NM_016966, NM_031620, NM_006623
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of PHGDH antibody
- Kit Component:
- - KN313216G1, Phgdh gRNA vector 1 in pCas-Guide vector
- KN313216G2, Phgdh gRNA vector 2 in pCas-Guide vector
- KN313216D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration sup. ug/ml]
Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration sup. ug/ml]
PHGDH monoclonal antibody (M01), clone 4A3-1D6. Western Blot analysis of PHGDH expression in human liver.
PHGDH monoclonal antibody (M01), clone 4A3-1D6 Western Blot analysis of PHGDH expression in Jurkat ( Cat # L017V1 ).
Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody (M01), clone 4A3-1D6.Lane 1: PHGDH transfected lysate(56.7 KDa).Lane 2: Non-transfected lysate.
PHGDH monoclonal antibody (M01), clone 4A3-1D6. Western Blot analysis of PHGDH expression in A-431 ( Cat # L015V1 ).
Western Blot analysis of PHGDH expression in transfected 293T cell line (H00026227-T01) by PHGDH MaxPab polyclonal antibody.Lane 1: PHGDH transfected lysate(58.63 KDa).Lane 2: Non-transfected lysate.
PHGDH MaxPab polyclonal antibody. Western Blot analysis of PHGDH expression in Jurkat.
PHGDH MaxPab polyclonal antibody. Western Blot analysis of PHGDH expression in Raw 264.7.
PHGDH MaxPab rabbit polyclonal antibody. Western Blot analysis of PHGDH expression in human kidney.
Western Blot analysis of PHGDH expression in transfected 293T cell line (H00026227-T02) by PHGDH MaxPab polyclonal antibody.Lane 1: PHGDH transfected lysate(56.70 KDa).Lane 2: Non-transfected lysate.
(LEFT) Western blot analysis of PHGDH Antibody (N-term) (AP17635PU-N) in mouse brain tissue lysates (35ug/lane). PHGDH (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PHGDH (arrow) using rabbit polyclonal PHGDH Antibody (N-term) (AP17635PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2) .
Human 293T; WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T.; PHGDH antibody - middle region (ARP54801_P050) in Human 293T cells using Western Blot
Human 293T; WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate. PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T.; PHGDH antibody - middle region (ARP54802_P050) in Human 293T cells using Western Blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1 ~ 10 µg/ml]
Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody, clone 4A3-1D6.
Western Blot analysis of PHGDH expression in human liver with PHGDH monoclonal antibody, clone 4A3-1D6.
Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806763)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (RC203949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (RC203949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806876)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (RC203949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysatePHGDH is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 293T cell lysatePHGDH is supported by BioGPS gene expression data to be expressed in HEK293T
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: NIH/3T3 cells, A431 cells, Primary antibody: (PHGDH Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA351523 | PHGDH antibody |
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| Rabbit Polyclonal Anti-PHGDH Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
$335.00
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| OriGene Technologies, Inc. | |||||||
| AP17635PU-N | PHGDH (N-term) antibody |
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Rabbit | Hu | WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||