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PCCA antibody

Principal name

PCCA antibody

Alternative names for PCCA antibody

PCCase subunit alpha, Propionyl-CoA carboxylase alpha chain mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit alpha

SwissProt ID

P05165 (Human), P14882 (Rat), Q19842 (Caeel), Q91ZA3 (Mouse)

Gene ID

5095 (PCCA), 110821 (Pcca), 687008

Ncbi ID

NP_000273.2, NP_000273, AAH00140.1, NM_001178004, NP_659093.2, NM_144844, NM_019330, NM_000282, NM_001127692

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB)

Background of PCCA antibody

Kit Component:: - KN312872G1, Pcca gRNA vector 1 in pCas-Guide vector
- KN312872G2, Pcca gRNA vector 2 in pCas-Guide vector
- KN312872D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Ugarte,M., (er) Am. J. Hum. Genet. 81 (6) (2007) In press

PCCA MaxPab polyclonal antibody. Western Blot analysis of PCCA expression in human liver.

H00005095-B01P

Western Blot analysis of PCCA expression in transfected 293T cell line (H00005095-T01) by PCCA MaxPab polyclonal antibody.Lane 1: PCCA transfected lysate(77.33 KDa).Lane 2: Non-transfected lysate.

H00005095-B01P

Transfected 293T; WB Suggested Anti-PCCA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; PCCA antibody - N-terminal region (ARP33071_P050) in Transfected 293T cells using Western Blot

ARP33071_P050

PCCA Antibody (Center) (Cat. #AP53192PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

AP53192PU-N

PCCA Antibody (Center) (Cat. #AP53192PU-N) western blot analysis in A549 cell line lysates (35µg/lane).This demonstrates the PCCA antibody detected the PCCA protein (arrow).