MMP-13 antibody
Principal name
MMP-13 antibody
Alternative names for MMP-13 antibody
Collagenase 3, MMP13, Matrix metalloproteinase-13
Ncbi ID
NP_002418.1, 9606, NP_002418, NM_002427, NP_032633.1, NM_008607, NM_133530
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of MMP-13 antibody
Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.
General readings
Gomis-Rueth F.-X., et al (1996). J. Mol. Biol. 264:556-566
Lausch E., et al (2009). Am. J. Hum. Genet. 85:168-178
Western Blot analysis of MMP13 expression with Jurkat Cell Lysate.
Western Blot analysis of MMP13 expression in transfected 293T cell line by MMP13 monoclonal antibody (M07), clone 3B11.Lane 1: MMP13 transfected lysate(53.8 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of MMP13 over-expressed 293 cell line, cotransfected with MMP13 Validated Chimera RNAi ( Cat # H00004322-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MMP13 monoclonal antibody (M07), clone 3B11 (Cat # H00004322-M07 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged MMP13 is approximately 1ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes of MMP-13 antibody (Cat.-No.: AP06226PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of MMP-13 antibody (Cat.-No.: AP06226PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate treated with IL-1β
Lane2:sp2/0 whole cell lysate treated with IL-1β
Lane3:H9C2 whole cell lysate treated with IL-1β
Lane1:Hela whole cell lysate treated with IL-1β
Lane2:sp2/0 whole cell lysate treated with IL-1β
Lane3:H9C2 whole cell lysate treated with IL-1β
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Formalin-fixed and paraffin-embedded human hepatocellular cancer tissue reacted with MMP13 polyclonal antibody ( Cat # PAB1983 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot analysis of MMP13 expression in transfected 293T cell line (H00004322-T01) by MMP13 MaxPab polyclonal antibody.Lane 1: MMP13 transfected lysate(51.81 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MMP13 expression in transfected 293T cell line (H00004322-T01) by MMP13 MaxPab polyclonal antibody.Lane 1: MMP13 transfected lysate(53.80 KDa).Lane 2: Non-transfected lysate.
(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of MMP13(arrow) using rabbit polyclonal MMP13 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2)
Western blot analysis of Mmp13 expression in Jurkat cell lysate.
Human; Sample Type: 1. Normal HUman Cartilage (50ug)2. Human Osteoarthritis cartilage (50ug)3. Human Osteoarthritis chondrocytes (50ug)4. Human Osteoarthritis chondrocytes +IL-1 (50ug)Primary Dilution: 1:2000Secondary Antibody: HRP conjugated anti-rabbitSecondary Dilution: 1:5000Image Submitted By: Mukundan AtturNYU Hospital for Joint Disease; MMP13 antibody - middle region (ARP56350_P050) in Human cells using Western Blot
Equine; Sample Type: Equine Cartilage Explants Primary Dilution: 1:1000 Secondary Antibody: Bio-Rad 170-5046 Secondary Dilution: 1:100,000Image Submitted By: Adam WilliamsUniversity of Nottingham; MMP13 antibody - middle region (ARP56350_P050) in Equine cells using Western Blot
Human Placenta; WB Suggested Anti-MMP13 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; MMP13 antibody - middle region (ARP56350_P050) in Human Placenta cells using Western Blot
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical analysis of MMP13 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MMP13 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of MMP13 expression in HeLa (A); SP2/0 (B); H9C2 (C); MCF7 (D); HT1080 (E); SCC4 (F) whole cell lysates.
Representative Standard Curve
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506688)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506688)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506688)
Anti-MMP13 mouse monoclonal antibody (TA506688) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506689)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506689)
Anti-MMP13 mouse monoclonal antibody (TA506689) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Anti-MMP13 mouse monoclonal antibody (TA506692) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506693)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506693)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506693)
Immunofluorescent staining of HeLa cells using anti-MMP13 mouse monoclonal antibody (TA506693).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506697)
Anti-MMP13 mouse monoclonal antibody (TA506697) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Anti-MMP13 mouse monoclonal antibody (TA506699) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506719)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506721)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506721)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506723)
Anti-MMP13 mouse monoclonal antibody (TA506723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
Immunofluorescent staining of HeLa cells using anti-MMP13 mouse monoclonal antibody (TA506723).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506803)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506803)
Anti-MMP13 mouse monoclonal antibody (TA506803) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Anti-MMP13 mouse monoclonal antibody (TA506804) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506806)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506806)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506806)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506806)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506806)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506806)
Anti-MMP13 mouse monoclonal antibody (TA506806) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13(RC224156).
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-MMP13 monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (RC224156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.
Predicted band size: 54kDa. Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate:40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human stomach and liver cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human stomach cancer tissue using MMP13 antibody at dilution 1/10, on the right is immunohistochemistry of paraffin-embedded Human liver cancer tissue using MMP13 antibody at dilution 1/10. (Original magnification: x100)
Western blot analysis of extracts of various cell lines, using MMP13 antibody.
Anti-MMP13 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MMP13 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MMP13 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MMP13 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP00230PU-N | MMP-13 antibody |
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Rabbit | Aff - Purified | Bov, Eq, Hu, Ms, Rb, Rt | IP, P, WB |
0.1 mg /
$420.00
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| OriGene Technologies GmbH | |||||||