LITAF antibody
Principal name
LITAF antibody
Alternative names for LITAF antibody
LITAF, PIG7, SIMPLE, LPS-induced TNF-alpha factor, p53-induced gene 7 protein, Lipopolysaccharide-induced tumor necrosis factor-alpha factor, Small integral membrane protein of lysosome/late endosome
SwissProt ID
P0C0T0 (Rat), Q6GMG8 (Danre), Q6P828 (Xentr), Q8QGW7 (Chick), Q99732 (Human), Q9JLJ0 (Mouse)
Ncbi ID
9606, NP_004853, NP_001129944, NP_001129944.1, NM_004862, NM_001136472, NM_001136473, NP_064364.1, NM_019980
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), EPair for Elisa (EPair), Immunoprecipitation (IP)
Background of LITAF antibody
- Kit Component:
- - KN309323G1, Litaf gRNA vector 1 in pCas-Guide vector
- KN309323G2, Litaf gRNA vector 2 in pCas-Guide vector
- KN309323D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Atucchi A, et al., (2006) Inflamm Bowel Dis 13(1):120–121.
Tang X, et al., (2005) Proc Natl Acad Sci USA 102(14):5132–5137.
Tang X, et al., (2003) Proc Natl Acad Sci USA 100(7):4096–4101.
Immunoperoxidase of purified MaxPab antibody to LITAF on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to LITAF on HeLa cell. [antibody concentration 10 ug/ml]
LITAF MaxPab polyclonal antibody. Western Blot analysis of LITAF expression in human placenta.
Western Blot analysis of LITAF expression in transfected 293T cell line (H00009516-T01) by LITAF MaxPab polyclonal antibody.Lane 1: LITAF transfected lysate(17.71 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of LITAF expression in transfected 293T cell line (H00009516-T02) by LITAF MaxPab polyclonal antibody.Lane 1: LITAF transfected lysate(17.10 KDa).Lane 2: Non-transfected lysate.
Flow cytometry analysis of LITAF in HeLa cell lin staining staining at 2-5ug for 1x10 5ug for 1x106 cells. The secondary antibody used goat anti -mouse IgG Alexa fluor 488 conjugate.
Western blot analysis: Recombinant Human LITAF protein (50 ng) and cell lysates (40 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LITAF (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant LITAF protein, Lane 2: HepG2 cell lysate, Lane 3: HeLa cell lysate, Lane 4: SW480 cell lysate.
Immunoprecipitation of LITAF transfected lysate using anti-LITAF MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LITAF MaxPab mouse polyclonal antibody (B01) (H00009516-B01).
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. TA308382 diluted at 1:1000
LITAF antibody [N1C3] detects LITAF protein at cytoplasm on placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. LITAF antibody [N1C3] (TA308382) dilution: 1:100.
Western blot analysis of extracts of various cell lines, using LITAF antibody.
Immunohistochemical of paraffin-embedded mouse lung using LITAF antibody at dilution of 1:200 (400x lens).
Immunohistochemical of paraffin-embedded human endometrial cancer using LITAF antibody at dilution of 1:200 (400x lens).
Immunohistochemical of paraffin-embedded human thyroid cancer using LITAF antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using LITAF antibody. Blue: DAPI for nuclear staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308382 | LITAF antibody |
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| Rabbit polyclonal antibody to LITAF (lipopolysaccharide-induced TNF factor) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
$415.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AM09081PU-N | LITAF antibody |
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 |
Mouse | IgG2b | AT5C10 | Purified | Hu | E, F, WB |
0.1 ml /
$330.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM09081PU-S | LITAF antibody |
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|
Mouse | IgG2b | AT5C10 | Purified | Hu | E, F, WB |
50 µl /
$220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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