HDAC9 antibody
Principal name
HDAC9 antibody
Alternative names for HDAC9 antibody
HDAC7B, HDRP, KIAA0744, MITR, Histone deacetylase 9, HD9, HD7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR
Ncbi ID
NP_001191073.1, 9606, NP_055522, NM_178423, NM_014707, NP_077038.2, NM_024124, NM_178425
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)
Background of HDAC9 antibody
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 may play a role in hematopoiesis.Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
General readings
Maltepe, E., et al. (2005) Development 132: 3393-3403.
Detection limit for recombinant GST tagged HDAC9 is approximately 0.1ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes ofHDAC9 antibody (Cat.-No.: AP06163PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of HDAC 9 antibody (Cat.-No.: AP06163PU-N) at 1/500 dilution
Lane1:LO2 whole cell lysate
Lane2:Mouse heart tissue lysate
Lane3:Rat heart tissue lysate
Lane1:LO2 whole cell lysate
Lane2:Mouse heart tissue lysate
Lane3:Rat heart tissue lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody ( top panel ) or a FLAG antibody ( bottom panel ) detecting a FLAG epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
Immunoblots for HDAC9 polyclonal antibody ( Cat # PAB2338 ) , Mirk, MyoD and tubulin proteins are shown for cytoplasmic ( Cyt ) and nuclear ( N ) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk ( Wt ) , kinase-inactive Mirk ( YF ) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
Both HDAC9 ( Cat # PAB2338 ) and HDAC9 ( Cat # PAB2339 ) polyclonal antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both polyclonal antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both polyclonal antibody can immunoprecipitate ( IP ) HDAC9 from HeLa-HDAC9 tranfected cells. ( Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL ) .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HDAC9 polyclonal antibody ( Cat # PAB2339 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Both anti-HDAC9 N-term ( Cat # PAB2238 ) and C-term ( Cat # PAB2239 ) polyclonal antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells.Top figure shows both polyclonal antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.Bottom figure shows that both polyclonal antibody can immunoprecipitate ( IP ) HDAC9 from HeLa-HDAC9 tranfected cells. ( Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL ) .
Human kidney; HDAC9 antibody - C-terminal region (ARP42788_T100) in Human kidney cells using Immunohistochemistry
Human Lung; . Rabbit Anti-HDAC9 Antibody. . Catalog Number:. ARP42788 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human A172; WB Suggested Anti-HDAC9 Antibody Titration: 1.25ug/ml. Positive Control: A172 cell lysate. HDAC9 is supported by BioGPS gene expression data to be expressed in A172.; HDAC9 antibody - C-terminal region (ARP42788_T100) in Human A172 cells using Western Blot
HCT116; Chromatin Immunoprecipitation (ChIP) Using HDAC9 antibody - C-terminal region (ARP42788_T100) and HCT116 Cells; HDAC9 antibody - C-terminal region (ARP42788_T100) in HCT116 cells using Chromatin Immunoprecitation
Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Figure 1. Both anti-HDAC9 N-term (AP11138PU-N) and C-term (AP11139PU-N) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top Figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom Figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).
Western blot analysis of HDAC9 in mouse small intestine tissue lysate. The antibody recognizes a ~110-140 kDa of HDAC9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.
Immunofluorescence of purified MaxPab antibody to HDAC9 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of HDAC9 expression in transfected 293T cell line (H00009734-T01) by HDAC9 MaxPab polyclonal antibody.Lane 1: HDAC9 transfected lysate(117.59 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-HDAC9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate; HDAC9 antibody - middle region (ARP42793_P050) in Human Jurkat cells using Western Blot
Immunohistochemical analysis of Histone Deacetylase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone Deacetylase 9 staining in Ramos cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Histone Deacetylase 9 expression in LO2 (A); mouse heart (B); rat heart (C); Ramos (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805257)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805257)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805257)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (RC215267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805308)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805308)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (RC215267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (RC215267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805323)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805323)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (RC215267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805328)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (RC215267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
Western blot analysis of extracts from HepG2 cells, using HDAC9 antibody (#TA314963).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC9 antibody (#TA314963).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using HDAC9 antibody (#TA314963).The picture on the right is treated with the synthesized peptide.
Western blot analysis of HDAC-9 in mouse small intestine tissue lysate.
Both anti-HDAC9 N-term (TA324378) and C-term (AP1109b) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.
Figure 1: Immunoblots for MITR (TA324378 HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a FLAG antibody (bottom panel) detecting a FLAG epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).
Western blot analysis of HDAC9 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on HepG2 cell lysate using HDAC9 Antibody
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC9 Antibody.
Western blot analysis of extracts of various cell lines, using HDAC9 antibody.
Western Blot: HDAC9 Antibody [TA336893] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-r
Immunohistochemistry-Paraffin: HDAC9 Antibody [TA336893] - Analysis of HDAC-9 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and TA336893 (bottom left, right) at 5 ug/ml.
Western Blot: HDAC9 [p Ser155] Antibody [TA336972] - Western blot analysis of phosphorylated HDAC9 in (A) recombinant fusion protein containing a phosphorylated serine at position155and (B) fusion protein containinugan unphosphorylated serine at position
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324378 | HDAC9 (N-term) antibody |
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| Rabbit polyclonal HDAC9 Antibody (N-term) | |||||||
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Rabbit | Ig | Purified | Hu | ICC/IF, IP, P, WB |
0.4 ml /
$415.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| AP11139PU-N | HDAC9 (C-term) antibody |
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Rabbit | Ig | Purified | Hu | IP, P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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