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GRASP antibody

Principal name

GRASP antibody

Alternative names for GRASP antibody

GRP1-associated scaffold protein, General receptor for phosphoinositides 1-associated scaffold protein

SwissProt ID

Q7Z6J2 (Human), Q8R4T5 (Rat), Q9JJA9 (Mouse)

Gene ID

56149 (Grasp), 192254, 160622 (GRASP)

Ncbi ID

10090, 10116, NM_181711, NP_620249.1, NP_062391.3, NP_859062.1, NM_019518, NM_138894

Available reactivities

Ms (Mouse), Rt (Rat), Bov (Bovine), Hu (Human), Can (Canine)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of GRASP antibody

Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domaincontaining protein that interacts with group 1 metabotropic glutamate receptors (mGluRs). The PDZ domaincontaining amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs. The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins. It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.

Western blot using Grasp polyclonal antibody ( Cat # PAB11330 ) to detect over-expressed Grasp in HEK293 cells ( lane 2, arrowhead ).Lane 1 shows the non-transfected control.The primary antibody at a 1 : 2,000 dilution.Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

PAB11330

Mouse brain lysate was immunoprecipitated with Grasp polyclonal antibody ( Cat # PAB11330 ).A blot was prepared and probed with affinity purified anti-Grasp.Lane 1 is wild-type brain lysate; Lane 2 is Grasp knock-out brain lysate.The membrane was probed with the primary antibody at a 1:1,000 dilution.Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

PAB11330

Western blot of rat cerebellar lysate showing the specific immunolabeling of the ~95k Grasp protein. Using Grasp polyclonal antibody ( Cat # PAB9619 ).

PAB9619

Western blot of rat cerebellar lysate showing the specific immunolabeling of the ~95k GRASP protein.

TA309160

Anti-GRASP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

TA316870

Western blot using affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

TA319490

Mouse brain lysate was immunoprecipitated with anti-Tamalin antiserum. A blot was prepared and probed with affinity purified anti-Tamalin. Lane 1 is wild-type brain lysate; Lane 2 is Tamalin knock-out brain lysate. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

TA319490

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA309160	GRASP (C-term) antibody
	Rabbit Anti-GRASP Antibody
	Rabbit	IgG	Aff - Purified	Bov, Can, Hu, Ms, Rt	WB	0.1 ml / $405.00
						OriGene Technologies, Inc.

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