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FANCC antibody

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Principal name

FANCC antibody

Alternative names for FANCC antibody

Fanconi anemia group C protein, FAC, FACC

SwissProt ID

O19104 (Bovin), O35870 (Rat), P50652 (Mouse), Q00597 (Human)

Gene ID

2176 (FANCC), 24361 (Fancc)

Ncbi ID

9606, NP_000127.2, NP_000127, NM_012557

Available reactivities

Hu (Human), Mky (Monkey), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of FANCC antibody

FANCC (also called Protein FACC or Fanconi Anemia Group C protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCC may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCC belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is ubiquitously expressed. Defects in FANCC are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopaenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

General readings

1. Hirano,S., Yamamoto,K., Ishiai,M., Yamazoe,M., Arakawa,H., Buerstedde,J.M., Enomoto,T., Takeda,S., Thompson,L.H. and Takata,M. (2005)
Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLM. EMBO J. 24 (2), 418-427.
2. Mi,J. and Kupfer,G.M. (2005) The Fanconi anemia core complex associates with chromatin during S phase. Blood 105 (2), 759-766.
3. Zhang,X., Li,J., Sejas,D.P., Rathbun,K.R., Bagby,G.C. and Pang,Q. (2004) The Fanconi anemia proteins functionally interact with the protein
kinase regulated by RNA (PKR). J. Biol. Chem. 279 (42), 43910-43919.
4. Niedzwiedz,W., Mosedale,G., Johnson,M., Ong,C.Y., Pace,P. and Patel,K.J. (2004) The Fanconi anaemia gene FANCC promotes
homologous recombination and error-prone DNA repair. Mol. Cell 15 (4), 607-620.
5. Zanier,R., Briot,D., Dugas du Villard,J.A., Sarasin,A. and Rosselli,F. (2004) Fanconi anemia C gene product regulates expression of genes
involved in differentiation and inflammation. Oncogene 23 (29), 5004-5013.
6. Brodeur,I., Goulet,I., Tremblay,C.S., Charbonneau,C., Delisle,M.C., Godin,C., Huard,C., Khandjian,E.W., Buchwald,M., Levesque,G. and
Carreau,M. (2004) Regulation of the Fanconi anemia group C protein through proteolytic modification. J. Biol. Chem. 279 (6), 4713-4720.
7. Strathdee,C.A., Duncan,A.M. and Buchwald,M. (1992) Evidence for at least four Fanconi anaemia genes including FACC on chromosome 9.
Nat. Genet. 1 (3), 196-198.

H00002176-M01 - FANCC
Western Blot analysis of FANCC expression in transfected 293T cell line by FANCC monoclonal antibody (M01), clone 6E7.Lane 1: FANCC transfected lysate(63.4 KDa).Lane 2: Non-transfected lysate.
H00002176-B01 - FANCC
Western Blot analysis of FANCC expression in transfected 293T cell line (H00002176-T01) by FANCC MaxPab polyclonal antibody.Lane 1: FANCC transfected lysate(61.38 KDa).Lane 2: Non-transfected lysate.
H00002176-B01 - FANCC
FANCC MaxPab polyclonal antibody. Western Blot analysis of FANCC expression in Hela NE.
AP07582PU-N - FANCC
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE)on Brain, cortex.
H00002176-B01P - FANCC
Western Blot analysis of FANCC expression in transfected 293T cell line (H00002176-T01) by FANCC MaxPab polyclonal antibody.Lane 1: FANCC transfected lysate(61.38 KDa).Lane 2: Non-transfected lysate.
H00002176-B01P - FANCC
FANCC MaxPab polyclonal antibody. Western Blot analysis of FANCC expression in Hela NE.
H00002176-D01P - FANCC
FANCC MaxPab rabbit polyclonal antibody. Western Blot analysis of FANCC expression in human kidney.
H00002176-D01P - FANCC
Western Blot analysis of FANCC expression in transfected 293T cell line (H00002176-T01) by FANCC MaxPab polyclonal antibody.Lane 1: FANCC transfected lysate(63.40 KDa).Lane 2: Non-transfected lysate.
PAB10046 - FANCC
Western blot using FANCC polyclonal antibody ( Cat # PAB10046 ) shows detection of a band at ~63 kDa ( arrowhead ) corresponding to FANCC present in a HeLa whole cell lysate.The identity of the lower molecular weight band is unknown.Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to1:1,500 in PBS supplemented with 1% normal goatserum and 0.1% BLOTTO.The membrane was washed and reacted with a 1:10,000 dilution ofIRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).Molecular weight estimation was made bycomparison to prestained MW markers ( indicated at left ).IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
PAB12372 - FANCC
Western blot analysis of FANCC in transfected COS-1 cell lysate using FANCC polyclonal antibody ( Cat # PAB12372 ).
AP23096PU-N - FANCC
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23096PU-N - FANCC
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23096PU-N - FANCC
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, merged with DNA probe, using FANCC antibody at 1:200 dilution.
AP23096PU-N - FANCC
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FANCC antibody at 1:200 dilution.
AP23096PU-N - FANCC
Western Blot Image: Sample (30 µg of whole cell lysate). A: H1299. 7.5% SDS PAGE. FANCC antibody diluted at 1:1000
AP23096PU-N - FANCC
Western Blot Image: Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. FANCC antibody. FANCC antibody diluted at 1:1000.
AP23579PU-N - FANCC
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23579PU-N - FANCC
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
TA590808 - FANCC
Immunohistochemical staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590808 - FANCC
Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm & cytoskeleton (intermediate filaments).This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590808 - FANCC
ELISA: FANCC Antibody
TA301760 - FANCC
Detection of FANC C in transfected COS1 cell lysate
TA308740 - FANCC
Sample (30 ug of whole cell lysate). . A: NIH-3T3. . 7.5% SDS PAGE. . FANCC antibody. . TA308740 diluted at 1:1000.
TA308740 - FANCC
FANCC antibody detects FANCC protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with FANCC antibody (TA308740) diluted by 1:1000
TA308740 - FANCC
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using FANCC(TA308740) antibody at 1:100 dilution.
TA308740 - FANCC
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FANCC(TA308740) antibody at 1:200 dilution.
TA309655 - FANCC
Western Blot: FANCC Antibody (8F3) - Western blot on hydroxyurea (HU) synchronized 293 FANCC E2 cells.
TA319294 - FANCC
WB using Anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate. The identity of the lower molecular weight band is unknown. The primary antibody was diluted to 1:1,500. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10,000. Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).
TA323732 - FANCC
Predicted band size: 63 kDa . Positive control: Brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA325105 - FANCC
Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody (C-term)(Cat. #TA325105).TA325105 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
TA325105 - FANCC
FANCC Antibody (C-term)(Cat. #TA325105) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FANCC Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
TA325105 - FANCC
Immunofluorescent analysis of U251 cells, using FANCC Antibody (C-term) (Cat. #TA325105). TA325105 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
TA325105 - FANCC
FANCC Antibody (C-term) (Cat. #TA325105) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA327186 - FANCC
Western blot analysis of extracts of brain cell lines, using FANCC antibody.
TA327186 - FANCC
Immunofluorescence analysis of U2OS cell using FANCC antibody. Blue: DAPI for nuclear staining.
TA336732 - FANCC
Western Blot: FANCC Antibody [TA336732] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2.
TA336733 - FANCC
Western Blot: FANCC Antibody [TA336733] - Western blot analysis of FANCC expression in 1) NTera2 and 2) Cos 7 whole cell lysates.
TA336733 - FANCC
Western Blot: FANCC Antibody [TA336733] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2. anti-FANCC [TA336733].
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FANCC (1-275) antibody
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / $425.00
  OriGene Technologies GmbH
+5 additional images

Highly validated antibody!

FANCC antibody
  Rabbit Polyclonal FANCC Antibody  
Immunohistochemical staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / $409.00
  OriGene Technologies, Inc.
+2 additional images

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