EHMT1 antibody
Principal name
EHMT1 antibody
Alternative names for EHMT1 antibody
EUHMTASE1, GLP, KIAA1876, KMT1D, Eu-HMTase1, G9a-like protein 1, GLP, GLP1, H3-K9-HMTase 5, Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1, Histone H3-K9 methyltransferase 5, Lysine N-methyltransferase 1D
Ncbi ID
9606, NP_001138999.1, NP_001012536.2, NM_024757, NM_001109686, NM_172545, NM_001109687, NM_001145527
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunohistochemistry (IHC), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P)
Background of EHMT1 antibody
- Kit Component:
- - KN305078G1, Ehmt1 gRNA vector 1 in pCas-Guide vector
- KN305078G2, Ehmt1 gRNA vector 2 in pCas-Guide vector
- KN305078D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of EHMT1 ( arrow ) using rabbit EHMT1 polyclonal antibody ( Cat # PAB2181 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the EHMT1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of EHMT1 ( arrow ) using rabbit EHMT1 polyclonal antibody ( Cat # PAB2182 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the EHMT1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of EHMT1 ( arrow ) using rabbit EHMT1 polyclonal antibody ( Cat # PAB2183 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the EHMT1 gene ( Lane 2 ) ( Origene Technologies ) .
EHMT1 monoclonal antibody (M04), clone 1H2 Western Blot analysis of EHMT1 expression in MES-SA/Dx5 ( Cat # L021V1 ).
Immunofluorescence of monoclonal antibody to EHMT1 on HeLa cell. [antibody concentration 10 ug/ml]
EHMT1 monoclonal antibody (M05), clone 3G1 Western Blot analysis of EHMT1 expression in Hela NE ( Cat # L013V3 ).
EHMT1 monoclonal antibody (M09), clone 3C5. Western Blot analysis of EHMT1 expression in Raw 264.7
Immunofluorescence of purified MaxPab antibody to EHMT1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of EHMT1 expression in transfected 293T cell line (H00079813-T01) by EHMT1 MaxPab polyclonal antibody.Lane 1: EHMT1 transfected lysate(88.88 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of EUHMTASE (arrow) using Rabbit polyclonal EHMT1 Antibody (N-term) Cat.-No AP11092PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EUHMTASE gene (Lane 2) (Origene Technologies).
Western blot analysis of EUHMTASE (arrow) using EHMT1 Antibody (Center) Cat.-No AP11093PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EUHMTASE gene (Lane 2) (Origene Technologies).
Western blot analysis using GLP mouse mAb against GLP recombinant protein.
Western blot analysis of EHMT1 (arrow) using rabbit polyclonal EHMT1 Antibody (Center)(Cat.#TA302190). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2) (Origene Technologies).
Western blot analysis of EHMT1 (arrow) using rabbit polyclonal EHMT1 Antibody (C-term) (Cat#TA302191). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2) (Origene Technologies).
Western blot analysis of EHMT1 (arrow) using rabbit polyclonal EHMT1 Antibody (N-term) (Cat# TA302192). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry: EHMT1 Antibody [TA336827] - IHC analysis of EMHT1 in mouse prostate using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: EHMT1 Antibody [TA336827] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
WB using the antibody against Ehmt1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 138 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Ehmt1. The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:10,600.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302192 | EHMT1 (N-term) antibody |
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| Rabbit Polyclonal Antibody against EHMT1 (N-term) | |||||||
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
$415.00
|
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| OriGene Technologies, Inc. | |||||||
| AP11092PU-N | EHMT1 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies GmbH | |||||||